The dynamic interactions between chemotherapy drugs and plasmid DNA investigated by atomic force microscopy

CORC > 沈阳自动化研究所 > 中国科学院沈阳自动化研究所 > 机器人学研究室

	The dynamic interactions between chemotherapy drugs and plasmid DNA investigated by atomic force microscopy
	Li M(李密); Liu LQ(刘连庆); Xiao XB(肖秀斌); Xi N(席宁); Wang YC(王越超)
刊名	Science China Materials
	2017
卷号	60 期号:3 页码:269-278
关键词	AFM plasmid 化疗药物 DNA methotrexate arrows 甲氨蝶呤 length 原子力显微镜 purine
ISSN号	2095-8226
其他题名	基于AFM的化疗药物与质粒DNA间动态相互作用观测
通讯作者	刘连庆 ; 席宁
产权排序	1
中文摘要	The advent of atomic force microscopy (AFM) provides a powerful tool for imaging individual DNA molecules. Chemotherapy drugs are often related to DNAs. Though many specific drug-DNA interactions have been observed by AFM, knowledge about the dynamic interactions between chemotherapy drugs and plasmid DNAs is still scarce. In this work, AFM was applied to investigate the nanoscale interactions between plasmid DNAs and two commercial chemotherapy drugs (methotrexate and cisplatin). Plasmid DNAs were immobilized on mica which was coated by silanes in advance. AFM imaging distinctly revealed the dynamic changes of single plasmid DNAs after the stimulation of methotrexate and cisplatin. Geometric features of plasmid DNAs were extracted from AFM images and the statistical results showed that the geometric features of plasmid DNAs changed significantly after the stimulation of drugs. This research provides a novel idea to study the actions of chemotherapy drugs against plasmid DNAs at the single-molecule level.
WOS标题词	Science & Technology ; Technology
类目[WOS]	Materials Science, Multidisciplinary
研究领域[WOS]	Materials Science
关键词[WOS]	SINGLE-MOLECULE ; CANCER-CELLS ; MEASUREMENTS REVEAL ; PERSISTENCE LENGTH ; MEMBRANE-PROTEINS ; SPECTROSCOPY ; CISPLATIN ; TEMPERATURE ; DEPENDENCE ; MORPHOLOGY
收录类别	SCI ; CSCD
语种	英语
WOS记录号	WOS:000398129000008
内容类型	期刊论文
源URL	[http://ir.sia.cn/handle/173321/20246]
专题	沈阳自动化研究所_机器人学研究室
推荐引用方式 GB/T 7714	Li M,Liu LQ,Xiao XB,et al. The dynamic interactions between chemotherapy drugs and plasmid DNA investigated by atomic force microscopy[J]. Science China Materials,2017,60(3):269-278.
APA	Li M,Liu LQ,Xiao XB,Xi N,&Wang YC.(2017).The dynamic interactions between chemotherapy drugs and plasmid DNA investigated by atomic force microscopy.Science China Materials,60(3),269-278.
MLA	Li M,et al."The dynamic interactions between chemotherapy drugs and plasmid DNA investigated by atomic force microscopy".Science China Materials 60.3(2017):269-278.