Overexpression of glucose-6-phosphate dehydrogenase enhanced the polyunsaturated fatty acid composition of Aurantiochytrium sp SD116

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 代谢物组学团队

	Overexpression of glucose-6-phosphate dehydrogenase enhanced the polyunsaturated fatty acid composition of Aurantiochytrium sp SD116
	Cui, Gu-Zhen 1; Ma, Zengxin 1,2; Liu, Ya-Jun 1; Feng, Yingang 1; Sun, Zhijie 1; Cheng, Yurong 1,2; Song, Xiaojin 1; Cui, Qiu 1
刊名	ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
	2016-11-01
卷号	19 页码:138-145
关键词	Aurantiochytrium Polyunsaturated fatty acid Glucose-6-phosphate dehydrogenase NADPH
英文摘要	Aurantiochytrium sp. (Thraustochytriaceae, Stramenopiles) is a highly promising polyunsaturated fatty acid (PUFA) resource due to its high docosahexaenoic acid (DHA) production. The reducing agent NADPH is essential for the production of PUFA and other fatty acids, and glucose-6-phosphate dehydrogenase (G6PDH) is one of the most important enzymes for NADPH regeneration in Aurantiochytrium. To investigate whether PUFA production could be enhanced by stimulating G6PDH, a recombinant strain, termed SD116::G6PDH, was constructed by overexpressing G6PDH in Aurantiochytriumsp. SD116. Although decreased cell growth and fatty acid production were observed, G6PDH overexpression changed the fatty acid profile and enhanced the proportion of PUFA in lipids by 10.6%. These results confirmed the positive effect of G6PDH activity on PUFA production and suggested that the manipulation of NADPH production is a promising strategy for increasing PUFA production in Aurantiochytrium. (C) 2016 Elsevier B.V. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology
关键词[WOS]	GENE PHYLOGENY THRAUSTOCHYTRIACEAE ; DOCOSAHEXAENOIC ACID ; MALIC ENZYME ; MICROALGA SCHIZOCHYTRIUM ; TAXONOMIC REARRANGEMENT ; TRANSGENE EXPRESSION ; MUCOR-CIRCINELLOIDES ; LIPID-METABOLISM ; SYSTEM ; DHA
收录类别	SCI
语种	英语
WOS记录号	WOS:000390001400017
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/9205]
专题	青岛生物能源与过程研究所_代谢物组学团队
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao Engn Lab Single Cell Oil, Shandong Prov Key Lab Energy Genet,Key Lab Biofue, Qingdao, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Cui, Gu-Zhen,Ma, Zengxin,Liu, Ya-Jun,et al. Overexpression of glucose-6-phosphate dehydrogenase enhanced the polyunsaturated fatty acid composition of Aurantiochytrium sp SD116[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2016,19:138-145.
APA	Cui, Gu-Zhen.,Ma, Zengxin.,Liu, Ya-Jun.,Feng, Yingang.,Sun, Zhijie.,...&Cui, Qiu.(2016).Overexpression of glucose-6-phosphate dehydrogenase enhanced the polyunsaturated fatty acid composition of Aurantiochytrium sp SD116.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,19,138-145.
MLA	Cui, Gu-Zhen,et al."Overexpression of glucose-6-phosphate dehydrogenase enhanced the polyunsaturated fatty acid composition of Aurantiochytrium sp SD116".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 19(2016):138-145.