An electrochemical aptasensor electrocatalyst for detection of thrombin

CORC > 苏州纳米技术与纳米仿生研究所 > 中国科学院苏州纳米技术与纳米仿生研究所 > 先进材料研究部 > 李清文团队

	An electrochemical aptasensor electrocatalyst for detection of thrombin
	Tian, R; Chen, XJ; Li, QW(李清文); Yao, C
刊名	ANALYTICAL BIOCHEMISTRY
	2016
卷号	500
通讯作者	Tian, R ; Yao, C
英文摘要	This work reports a novel signal amplification strategy based on three-dimensional ordered macroporous C-60-poly(3,4-ethylenedioxythiophene)-1-butyl-3-methylimidazolium hexafluorophosphate (3DOM C-60-PEDOT-[BMIm][BF6]) for ultrasensitive detection of thrombin by cascade catalysis of Au-PEDOT@SiO2 microspheres and alcohol dehydrogenase (ADH). Au-PEDOT@SiO2 microspheres were constructed not only as nanocarriers to anchor the large amounts of secondary thrombin aptamers but also as nano catalysts to catalyze the oxidation of ethanol efficiently. Significantly, the electrochemical signal was greatly enhanced based on cascade catalysis: First, ADH catalyzed the oxidation of ethanol to acetaldehyde with the concomitant generation of NADH in the presence of beta-nicotinamide adenine dinucleotide hydrate (NADI(+)). Then, gold nanoparticles (AuNPs) as nanocatalysts could effectively catalyze NADH to produce NAD(+) with the help of PEDOT as redox probe. Under the optimal conditions, the proposed aptasensor exhibits a linear range of 2 x 10(-13) to 2 x 10(-8) M with a low detection limit of 2 x 10(-14) M for thrombin detection and shows high sensitivity and good specificity. (C) 2016 Elsevier Inc. All rights reserved.
关键词[WOS]	HIGHLY SENSITIVE DETECTION ; GOLD NANOPARTICLES ; CARBON NANOTUBE ; AMPLIFICATION APTASENSOR ; DUAL-AMPLIFICATION ; SOLAR-CELLS ; ELECTRODE ; MICROSPHERES ; POLYANILINE ; QUADRUPLEX
收录类别	SCI
语种	英语
WOS记录号	WOS:000373968700015
内容类型	期刊论文
源URL	[http://ir.sinano.ac.cn/handle/332007/4699]
专题	苏州纳米技术与纳米仿生研究所_先进材料研究部_李清文团队
推荐引用方式 GB/T 7714	Tian, R,Chen, XJ,Li, QW,et al. An electrochemical aptasensor electrocatalyst for detection of thrombin[J]. ANALYTICAL BIOCHEMISTRY,2016,500.
APA	Tian, R,Chen, XJ,Li, QW,&Yao, C.(2016).An electrochemical aptasensor electrocatalyst for detection of thrombin.ANALYTICAL BIOCHEMISTRY,500.
MLA	Tian, R,et al."An electrochemical aptasensor electrocatalyst for detection of thrombin".ANALYTICAL BIOCHEMISTRY 500(2016).