Concentration gradient generator for H460 lung cancer cell sensitivity to resist the cytotoxic action of curcumin in microenvironmental pH conditions

CORC > 苏州纳米技术与纳米仿生研究所 > 中国科学院苏州纳米技术与纳米仿生研究所 > 纳米仿生研究部 > 聂富强团队

	Concentration gradient generator for H460 lung cancer cell sensitivity to resist the cytotoxic action of curcumin in microenvironmental pH conditions
	Hu, Chunfei(胡春飞); Lin, Yu-Sheng(刘玉胜); Chen, Hongmei(陈红梅); Liu, Jingjing; Nie, Fuqiang(聂富强)
刊名	RSC ADVANCES
	2016
卷号	6 期号:109
通讯作者	Lin, YS ; Chen, HM ; Nie, FQ(聂富强)
英文摘要	We proposed and demonstrated a concentration gradient generator (CGG) to resist H460 lung cancer cells using curcumin in microenvironmental pH conditions. The CGG was designed as the integration of one-by-two and one-by-three fluidic channels to effectively generate a different concentration gradually. In cell biology, this CGG device was realized to test its operability and practicability. Therein, H460 lung cancer cells could be restrained by the injection of different curcumin concentrations. At a pH = 6.5 microenvironment, the apoptosis rate (AR) of H460 lung cancer cells was not obvious until the curcumin concentration was increased to 57.2 mg mL(-1), at which the AR was 47.8%. At pH = 7.4 and 8.5 microenvironments, the ARs of the H460 lung cancer cells were 54.5% and 93.8%, respectively, at a curcumin concentration of 57.2 mg mL(-1.) In the range of curcumin concentration of 57.2-100 mg mL(-1), the average ARs of the H460 lung cancer cells were 53.6%, 58.4%, and 98.0% for pH = 6.5, 7.4, and 8.5 microenvironments, respectively. The resistant efficiency of H460 lung cancer cells could be enhanced substantially at a pH = 8.5 microenvironmental condition. These results pave a way for the use of CGGs in cell biology application.
关键词[WOS]	MICROFLUIDIC DEVICE ; CHEMOTAXIS ; MIGRATION ; CULTURE ; DIFFERENTIATION ; PROGRESSION ; APOPTOSIS ; MEMBRANE ; NETWORKS ; INVASION
收录类别	SCI ; EI
语种	英语
WOS记录号	WOS:000389342400026
内容类型	期刊论文
源URL	[http://ir.sinano.ac.cn/handle/332007/4859]
专题	苏州纳米技术与纳米仿生研究所_纳米仿生研究部_聂富强团队
推荐引用方式 GB/T 7714	Hu, Chunfei,Lin, Yu-Sheng,Chen, Hongmei,et al. Concentration gradient generator for H460 lung cancer cell sensitivity to resist the cytotoxic action of curcumin in microenvironmental pH conditions[J]. RSC ADVANCES,2016,6(109).
APA	Hu, Chunfei,Lin, Yu-Sheng,Chen, Hongmei,Liu, Jingjing,&Nie, Fuqiang.(2016).Concentration gradient generator for H460 lung cancer cell sensitivity to resist the cytotoxic action of curcumin in microenvironmental pH conditions.RSC ADVANCES,6(109).
MLA	Hu, Chunfei,et al."Concentration gradient generator for H460 lung cancer cell sensitivity to resist the cytotoxic action of curcumin in microenvironmental pH conditions".RSC ADVANCES 6.109(2016).