Responses of the Proteome and Metabolome in Livers of Zebrafish Exposed Chronically to Environmentally Relevant Concentrations of Microcystin-LR

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 水生生物多样性与资源保护研究中心 > 期刊论文

	Responses of the Proteome and Metabolome in Livers of Zebrafish Exposed Chronically to Environmentally Relevant Concentrations of Microcystin-LR
	Chen, Liang 1,2; Hu, Yufei 1,2; He, Jun 1; Chen, Jun 1; Giesy, John P.3,4,5,6; Xie, Ping 1
刊名	ENVIRONMENTAL SCIENCE & TECHNOLOGY
	2017-01-03
卷号	51 期号:1 页码:596-607
ISSN号	0013-936X
英文摘要	In this study, for the first time, changes in expressions of proteins and profiles of metabolites in liver of the small, freshwater fish Danio rerio (zebrafish) were investigated after long-term exposure to environmentally relevant concentrations of microcystin-LR (MC-LR). Male zebrafish were exposed via water to 1 or 10 mu g MC-LR/L for 90 days, and iTRAQ-based proteomics and H-1 NMR-based metabolomics were employed. Histopathological observations showed that MC-LR caused damage to liver, and the effects were more pronounced in fish exposed to 10 mu g MC-LR/L. Metabolomic analysis also showed alterations of hepatic function, which included changes in a number of metabolic pathways, including small molecules involved in energy, glucose, lipids, and amino acids metabolism. Concentrations of lactate were significantly greater in individuals exposed to MC-LR than in unexposed controls. This indicated a shift toward anaerobic metabolism, which was confirmed by impaired respiration in mitochondria. Proteomics revealed that MC-LR significantly influenced multiple proteins, including those involved in folding of proteins and metabolism. Endoplasmic reticulum stress contributed to disturbance of metabolism of lipids in liver of zebrafish exposed to MC-LR. Identification of proteins and metabolites in liver of zebrafish responsive to MC-LR provides insights into mechanisms of chronic toxicity of MCs.
WOS标题词	Science & Technology ; Technology ; Life Sciences & Biomedicine
类目[WOS]	Engineering, Environmental ; Environmental Sciences
研究领域[WOS]	Engineering ; Environmental Sciences & Ecology
关键词[WOS]	ENDOPLASMIC-RETICULUM STRESS ; FISH ORYZIAS-LATIPES ; NF-KAPPA-B ; OXIDATIVE STRESS ; DANIO-RERIO ; MEDAKA FISH ; MOLECULAR-MECHANISMS ; 1ST IDENTIFICATION ; INDUCED APOPTOSIS ; AQUATIC ANIMALS
收录类别	SCI
语种	英语
WOS记录号	WOS:000391346900066
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/28861]
专题	水生生物研究所_水生生物多样性与资源保护研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Univ Saskatchewan, Dept Vet Biomed Sci, Saskatoon, SK S7N 5B3, Canada 4.Univ Saskatchewan, Toxicol Ctr, Saskatoon, SK S7N 5B3, Canada 5.Univ Hong Kong, Sch Biol Sci, Hong Kong, Hong Kong, Peoples R China 6.Nanjing Univ, Sch Environm, State Key Lab Pollut Control & Resource Reuse, Nanjing 210089, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Chen, Liang,Hu, Yufei,He, Jun,et al. Responses of the Proteome and Metabolome in Livers of Zebrafish Exposed Chronically to Environmentally Relevant Concentrations of Microcystin-LR[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2017,51(1):596-607.
APA	Chen, Liang,Hu, Yufei,He, Jun,Chen, Jun,Giesy, John P.,&Xie, Ping.(2017).Responses of the Proteome and Metabolome in Livers of Zebrafish Exposed Chronically to Environmentally Relevant Concentrations of Microcystin-LR.ENVIRONMENTAL SCIENCE & TECHNOLOGY,51(1),596-607.
MLA	Chen, Liang,et al."Responses of the Proteome and Metabolome in Livers of Zebrafish Exposed Chronically to Environmentally Relevant Concentrations of Microcystin-LR".ENVIRONMENTAL SCIENCE & TECHNOLOGY 51.1(2017):596-607.