Simultaneous Detection of Dual Nucleic Acids Using a SERS-Based Lateral Flow Assay Biosensor

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室

	Simultaneous Detection of Dual Nucleic Acids Using a SERS-Based Lateral Flow Assay Biosensor
	Wang, Xiaokun ; Choi, Namhyun ; Cheng, Ziyi ; Ko, Juhui ; Chen, Lingxin; Choo, Jaebum
刊名	ANALYTICAL CHEMISTRY
	2017-01-17
卷号	89 期号:2 页码:1163-1169
ISSN号	0003-2700
通讯作者	Choo, J (reprint author), Hanyang Univ, Dept Bionano Engn, Ansan 426791, South Korea.
产权排序	[Wang, Xiaokun; Choi, Namhyun; Cheng, Ziyi; Ko, Juhui; Choo, Jaebum] Hanyang Univ, Dept Bionano Engn, Ansan 426791, South Korea; [Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
英文摘要	A new class of surface-enhanced Raman scattering (SERS)-based lateral flow assay (LFA) biosensor has been developed for the simultaneous detection of dual DNA markers. The LFA strip in this sensor was composed of two test lines and one control line. SERS nano tags labeled with detection DNA probes were used for quantitative evaluation of dual DNA markers with high sensitivity. Target DNA, associated with Kaposis sarcoma-associated herpesvirus (KSHV) and bacillary angiomatosis (BA), were tested to validate the detection capability of this SERS-based LFA strip. Characteristic peak intensities of SERS nano tags on two test lines were used for quantitative evaluations of KSHV and BA. The limits of detection for KSHV and BA, determined from our SERS-based LFA sensing platform, were estimated to be 0.043 and 0.074 pM, respectively. These values indicate approximately 10000 times higher sensitivity than previously reported values using the aggregation-based colorimetric method. We believe that this is the first report of simultaneous detection of two different DNA mixtures using a SERS-based LFA platform. This novel detection technique is also a promising multiplex DNA sensing platform for early disease diagnosis.
研究领域[WOS]	Chemistry
关键词[WOS]	MULTICOLOR QUANTUM DOTS ; KAPOSIS-SARCOMA ; BACILLARY ANGIOMATOSIS ; QUANTITATIVE DETECTION ; TEST STRIP ; IMMUNOASSAY ; GOLD ; POINT ; AIDS ; NANOPARTICLES
收录类别	SCI
语种	英语
WOS记录号	WOS:000392458100021
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/20230]
专题	烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.Hanyang Univ, Dept Bionano Engn, Ansan 426791, South Korea 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xiaokun,Choi, Namhyun,Cheng, Ziyi,et al. Simultaneous Detection of Dual Nucleic Acids Using a SERS-Based Lateral Flow Assay Biosensor[J]. ANALYTICAL CHEMISTRY,2017,89(2):1163-1169.
APA	Wang, Xiaokun,Choi, Namhyun,Cheng, Ziyi,Ko, Juhui,Chen, Lingxin,&Choo, Jaebum.(2017).Simultaneous Detection of Dual Nucleic Acids Using a SERS-Based Lateral Flow Assay Biosensor.ANALYTICAL CHEMISTRY,89(2),1163-1169.
MLA	Wang, Xiaokun,et al."Simultaneous Detection of Dual Nucleic Acids Using a SERS-Based Lateral Flow Assay Biosensor".ANALYTICAL CHEMISTRY 89.2(2017):1163-1169.