Fluorometric determination of the antibiotic kanamycin by aptamer-induced FRET quenching and recovery between MoS2 nanosheets and carbon dots | |
Wang, Yashan1; Ma, Tiancong2; Ma, Shuyue3; Liu, Yongjun1; Tian, Yaping1; Wang, Ruinan1; Jiang, Yanbin1; Hou, Dongjun1; Wang, Jianlong3 | |
刊名 | MICROCHIMICA ACTA |
2017 | |
卷号 | 184期号:1页码:203-210 |
关键词 | Veterinary antibiotic Aptasensor Molybdenum disulfide MoS2 Exfoliation Fluorescence resonance energy transfer (FRET) Milk analysis |
英文摘要 | A rapid and sensitive aptamer-based assay is described for kanamycin, a veterinary antibiotic with neurotoxic side effects. It is based on a novel FRET pair consisting of fluorescent carbon dots and layered MoS2. This donor-acceptor pair (operated at excitation/emission wavelengths of 380/440 nm) shows fluorescence recovery efficiencies reaching 93 %. By taking advantages of aptamer-induced fluorescence quenching and recovery, kanamycin can be quantified in the of 4-25 mu M concentration range, with a detection limit of 1.1 mu M. The method displays good specificity and was applied to the determination of kanamycin in spiked milk where it gave recoveries ranging from 85 % to 102 %, demonstrating that the method serves as a promising tool for the rapid detection of kanamycin in milk and other animal-derived foodstuff. |
收录类别 | SCI |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://ir.iccas.ac.cn/handle/121111/36431] |
专题 | 化学研究所_其它 |
作者单位 | 1.China Anim Dis Control Ctr, Minist Agr, 20 Maizidian St, Beijing 100125, Peoples R China 2.Chinese Acad Sci, Inst Chem, Bei Yi Jie 2, Beijing 100190, Peoples R China 3.Northwest A&F Univ, Coll Food Sci & Engn, Yangling 712100, Shaanxi, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, Yashan,Ma, Tiancong,Ma, Shuyue,et al. Fluorometric determination of the antibiotic kanamycin by aptamer-induced FRET quenching and recovery between MoS2 nanosheets and carbon dots[J]. MICROCHIMICA ACTA,2017,184(1):203-210. |
APA | Wang, Yashan.,Ma, Tiancong.,Ma, Shuyue.,Liu, Yongjun.,Tian, Yaping.,...&Wang, Jianlong.(2017).Fluorometric determination of the antibiotic kanamycin by aptamer-induced FRET quenching and recovery between MoS2 nanosheets and carbon dots.MICROCHIMICA ACTA,184(1),203-210. |
MLA | Wang, Yashan,et al."Fluorometric determination of the antibiotic kanamycin by aptamer-induced FRET quenching and recovery between MoS2 nanosheets and carbon dots".MICROCHIMICA ACTA 184.1(2017):203-210. |
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