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Fluorometric determination of the antibiotic kanamycin by aptamer-induced FRET quenching and recovery between MoS2 nanosheets and carbon dots
Wang, Yashan1; Ma, Tiancong2; Ma, Shuyue3; Liu, Yongjun1; Tian, Yaping1; Wang, Ruinan1; Jiang, Yanbin1; Hou, Dongjun1; Wang, Jianlong3
刊名MICROCHIMICA ACTA
2017
卷号184期号:1页码:203-210
关键词Veterinary antibiotic Aptasensor Molybdenum disulfide MoS2 Exfoliation Fluorescence resonance energy transfer (FRET) Milk analysis
英文摘要A rapid and sensitive aptamer-based assay is described for kanamycin, a veterinary antibiotic with neurotoxic side effects. It is based on a novel FRET pair consisting of fluorescent carbon dots and layered MoS2. This donor-acceptor pair (operated at excitation/emission wavelengths of 380/440 nm) shows fluorescence recovery efficiencies reaching 93 %. By taking advantages of aptamer-induced fluorescence quenching and recovery, kanamycin can be quantified in the of 4-25 mu M concentration range, with a detection limit of 1.1 mu M. The method displays good specificity and was applied to the determination of kanamycin in spiked milk where it gave recoveries ranging from 85 % to 102 %, demonstrating that the method serves as a promising tool for the rapid detection of kanamycin in milk and other animal-derived foodstuff.
收录类别SCI
语种英语
内容类型期刊论文
源URL[http://ir.iccas.ac.cn/handle/121111/36431]  
专题化学研究所_其它
作者单位1.China Anim Dis Control Ctr, Minist Agr, 20 Maizidian St, Beijing 100125, Peoples R China
2.Chinese Acad Sci, Inst Chem, Bei Yi Jie 2, Beijing 100190, Peoples R China
3.Northwest A&F Univ, Coll Food Sci & Engn, Yangling 712100, Shaanxi, Peoples R China
推荐引用方式
GB/T 7714
Wang, Yashan,Ma, Tiancong,Ma, Shuyue,et al. Fluorometric determination of the antibiotic kanamycin by aptamer-induced FRET quenching and recovery between MoS2 nanosheets and carbon dots[J]. MICROCHIMICA ACTA,2017,184(1):203-210.
APA Wang, Yashan.,Ma, Tiancong.,Ma, Shuyue.,Liu, Yongjun.,Tian, Yaping.,...&Wang, Jianlong.(2017).Fluorometric determination of the antibiotic kanamycin by aptamer-induced FRET quenching and recovery between MoS2 nanosheets and carbon dots.MICROCHIMICA ACTA,184(1),203-210.
MLA Wang, Yashan,et al."Fluorometric determination of the antibiotic kanamycin by aptamer-induced FRET quenching and recovery between MoS2 nanosheets and carbon dots".MICROCHIMICA ACTA 184.1(2017):203-210.
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