A recognition-before-labeling strategy for sensitive detection of lung cancer cells with a quantum dot-aptamer complex

	A recognition-before-labeling strategy for sensitive detection of lung cancer cells with a quantum dot-aptamer complex
	Chunlei Wu; Jianbo Liu; Pengfei Zhang; Jing Li; Haining Ji; Xiaohai Yang; Kemin Wang
刊名	ANALYST
	2015
英文摘要	A highly specific recognition-before-labeling strategy has been developed for sensitive detection of non-small cell lung cancer A549 cells, by using fluorescent QDs as signal units and DNA aptamers as recognition elements. A QD-aptamer system used for cell imaging and bioanalysis mostly relies on the recognition-after-labeling strategy in which aptamers were firstly labeled with QDs and then the QD-aptamer conjugates as a whole were utilizedfor specific recognition. Here in our strategy, aptamers were used firstly to recognize target cells, and then fluorescent QDs were sequentially added to bind the aptamers and light the target cells. The proposed recognition-before-labeling strategy didn't require the complex process of QD functionalization, and avoided the possible impact on the aptamer configuration from steric hindrance. Meanwhile, QDs, with strong fluorescence and good photostability, also give this method a high signal-to-background ratio (S/B). The recognition-before-labeling strategy is simple and sensitive, suggesting a new methodfor in vitro diagnostic assays of cancer cells.
收录类别	SCI
原文出处	http://pubs.rsc.org/en/content/articlehtml/2015/an/c5an01145k
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/7351]
专题	深圳先进技术研究院_医药所
作者单位	ANALYST
推荐引用方式 GB/T 7714	Chunlei Wu,Jianbo Liu,Pengfei Zhang,et al. A recognition-before-labeling strategy for sensitive detection of lung cancer cells with a quantum dot-aptamer complex[J]. ANALYST,2015.
APA	Chunlei Wu.,Jianbo Liu.,Pengfei Zhang.,Jing Li.,Haining Ji.,...&Kemin Wang.(2015).A recognition-before-labeling strategy for sensitive detection of lung cancer cells with a quantum dot-aptamer complex.ANALYST.
MLA	Chunlei Wu,et al."A recognition-before-labeling strategy for sensitive detection of lung cancer cells with a quantum dot-aptamer complex".ANALYST (2015).