Fully integrated reflection-mode photoacoustic, two-photon, and second harmonic generation microscopy in vivo.

	Fully integrated reflection-mode photoacoustic, two-photon, and second harmonic generation microscopy in vivo.
	Song, Wei; Xu, Qiang; Zhang, Yang; Zhan, Yang; Zheng, Wei; Song, Liang
刊名	SCIENTIFIC REPORTS
	2016
英文摘要	The ability to obtain comprehensive structural and functional information from intact biological tissue in vivo is highly desirable for many important biomedical applications, including cancer and brain studies. Here, we developed a fully integrated multimodal microscopy that can provide photoacoustic (optical absorption), two-photon (fluorescence), and second harmonic generation (SHG) information from tissue in vivo, with intrinsically co-registered images. Moreover, using a delicately designed optical-acoustic coupling configuration, a high-frequency miniature ultrasonic transducer was integrated into a water-immersion optical objective, thus allowing all three imaging modalities to provide a high lateral resolution of similar to 290 nm with reflection-mode imaging capability, which is essential for studying intricate anatomy, such as that of the brain. Taking advantage of the complementary and comprehensive contrasts of the system, we demonstrated high-resolution imaging of various tissues in living mice, including microvasculature (by photoacoustics), epidermis cells, cortical neurons (by two-photon fluorescence), and extracellular collagen fibers (by SHG). The intrinsic image co-registration of the three modalities conveniently provided improved visualization and understanding of the tissue microarchitecture. The reported results suggest that, by revealing complementary tissue microstructures in vivo, this multimodal microscopy can potentially facilitate a broad range of biomedical studies, such as imaging of the tumor microenvironment and neurovascular coupling.
收录类别	SCI
原文出处	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5006040/
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/10505]
专题	深圳先进技术研究院_医工所
作者单位	SCIENTIFIC REPORTS
推荐引用方式 GB/T 7714	Song, Wei,Xu, Qiang,Zhang, Yang,et al. Fully integrated reflection-mode photoacoustic, two-photon, and second harmonic generation microscopy in vivo.[J]. SCIENTIFIC REPORTS,2016.
APA	Song, Wei,Xu, Qiang,Zhang, Yang,Zhan, Yang,Zheng, Wei,&Song, Liang.(2016).Fully integrated reflection-mode photoacoustic, two-photon, and second harmonic generation microscopy in vivo..SCIENTIFIC REPORTS.
MLA	Song, Wei,et al."Fully integrated reflection-mode photoacoustic, two-photon, and second harmonic generation microscopy in vivo.".SCIENTIFIC REPORTS (2016).