A Comprehensive Curation Shows the Dynamic Evolutionary Patterns of Prokaryotic CRISPRs

	A Comprehensive Curation Shows the Dynamic Evolutionary Patterns of Prokaryotic CRISPRs
	Guoqin Mai; Ruiquan Ge; Guoquan Sun; Qinghan Meng; Fengfeng Zhou
刊名	BioMed Research International
	2016
英文摘要	Motivation. Clustered regularly interspaced short palindromic repeat (CRISPR) is a genetic element with active regulation roles for foreign invasive genes in the prokaryotic genomes and has been engineered to work with the CRISPR-associated sequence (Cas) gene Cas9 as one of the modern genome editing technologies. Due to inconsistent definitions, the existing CRISPR detection programs seem to have missed some weak CRISPR signals. Results. This study manually curates all the currently annotated CRISPR elements in the prokaryotic genomes and proposes 95 updates to the annotations. A new definition is proposed to cover all the CRISPRs. The comprehensive comparison of CRISPR numbers on the taxonomic levels of both domains and genus shows high variations for closely related species even in the same genus. The detailed investigation of how CRISPRs are evolutionarily manipulated in the 8 completely sequenced species in the genus Thermoanaerobacter demonstrates that transposons act as a frequent tool for splitting long CRISPRs into shorter ones along a long evolutionary history.
收录类别	SCI
原文出处	http://www.hindawi.com/journals/bmri/2016/7237053/
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/10394]
专题	深圳先进技术研究院_医工所
作者单位	BioMed Research International
推荐引用方式 GB/T 7714	Guoqin Mai,Ruiquan Ge,Guoquan Sun,et al. A Comprehensive Curation Shows the Dynamic Evolutionary Patterns of Prokaryotic CRISPRs[J]. BioMed Research International,2016.
APA	Guoqin Mai,Ruiquan Ge,Guoquan Sun,Qinghan Meng,&Fengfeng Zhou.(2016).A Comprehensive Curation Shows the Dynamic Evolutionary Patterns of Prokaryotic CRISPRs.BioMed Research International.
MLA	Guoqin Mai,et al."A Comprehensive Curation Shows the Dynamic Evolutionary Patterns of Prokaryotic CRISPRs".BioMed Research International (2016).