Tumor Restrictive Suicide Gene Therapy for Glioma Controlled by the FOS Promoter

	Tumor Restrictive Suicide Gene Therapy for Glioma Controlled by the FOS Promoter
	Jianqing Pan; Hao Wang; Xinmin Liu; Jiliang Hu; Weijian Song; Jie Luo; Shan Jiang; Fei Yan; Baojin Zhai
刊名	PLOS ONE
	2015
英文摘要	Effective suicide gene delivery and expression are crucial to achieving successful effects in gene therapy. An ideal tumor-specific promoter expresses therapeutic genes in tumor cells with minimal normal tissue expression. We compared the activity of the FOS (FBJ murine osteosarcoma viral oncogene homolog) promoter with five alternative tumor-specific promoters in glioma cells and non-malignant astrocytes. The FOS promoter caused significantly higher transcriptional activity in glioma cell lines than all alternative promoters with the exception of CMV. The FOS promoter showed 13.9%, 32.4%, and 70.8% of the transcriptional activity of CMV in three glioma cell lines (U87, U251, and U373). Importantly, however, the FOS promoter showed only 1.6% of the transcriptional activity of CMV in normal astrocytes. We also tested the biologic activity of recombinant adenovirus containing the suicide gene herpes simplex virus thymidine kinase (HSV-tk) driven by the FOS promoter, including selective killing efficacy in vitro and tumor inhibition rate in vivo. Adenoviral-mediated delivery of the HSV-tk gene controlled by the FOS promoter conferred a cytotoxic effect on human glioma cells in vitro andin vivo. This study suggests that use of the FOS-tk adenovirus system is a promising strategy for glioma-specific gene therapy but still much left for improvement.
收录类别	SCI
原文出处	http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0143112
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/7174]
专题	深圳先进技术研究院_医工所
作者单位	PLOS ONE
推荐引用方式 GB/T 7714	Jianqing Pan,Hao Wang,Xinmin Liu,et al. Tumor Restrictive Suicide Gene Therapy for Glioma Controlled by the FOS Promoter[J]. PLOS ONE,2015.
APA	Jianqing Pan.,Hao Wang.,Xinmin Liu.,Jiliang Hu.,Weijian Song.,...&Baojin Zhai.(2015).Tumor Restrictive Suicide Gene Therapy for Glioma Controlled by the FOS Promoter.PLOS ONE.
MLA	Jianqing Pan,et al."Tumor Restrictive Suicide Gene Therapy for Glioma Controlled by the FOS Promoter".PLOS ONE (2015).