A novel molecular typing method of Mycobacteria based on DNA barcoding visualization

	A novel molecular typing method of Mycobacteria based on DNA barcoding visualization
	Bin Liu; Xiaotian Zhang; Honglan Huang; Ying Zhang; Fengfeng Zhou; Guoqing Wang
刊名	Journal of Clinical Bioinformatics
	2014
英文摘要	Different subtypes of Mycobacterium tuberculosis (MTB) may induce diverse severe human infections, and some of their symptoms are similar to other pathogenes, e.g. Nontuberculosis mycobacteria (NTM). So determination of mycobacterium subtypes facilitates the effective control of MTB infection and proliferation. This study exploits a novel DNA barcoding visualization method for molecular typing of 17 mycobacteria genomes published in the NCBI prokaryotic genome database. Three mycobacterium genes (Rv0279c, Rv3508 and Rv3514) from the PE/PPE family of MT Band were detected to best represent the inter-strain pathogenetic variations. An accurate and fast MTB substrain typing method was proposed based on the combination of the aforementioned three biomarker genes and the 16S rRNA gene. The protocol of establishing a bacterial substrain typing system used in this study may also be applied to the other pathogenes.
收录类别	其他
原文出处	http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3931916/pdf/2043-9113-4-4.pdf
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/5792]
专题	深圳先进技术研究院_医工所
作者单位	Journal of Clinical Bioinformatics
推荐引用方式 GB/T 7714	Bin Liu,Xiaotian Zhang,Honglan Huang,et al. A novel molecular typing method of Mycobacteria based on DNA barcoding visualization[J]. Journal of Clinical Bioinformatics,2014.
APA	Bin Liu,Xiaotian Zhang,Honglan Huang,Ying Zhang,Fengfeng Zhou,&Guoqing Wang.(2014).A novel molecular typing method of Mycobacteria based on DNA barcoding visualization.Journal of Clinical Bioinformatics.
MLA	Bin Liu,et al."A novel molecular typing method of Mycobacteria based on DNA barcoding visualization".Journal of Clinical Bioinformatics (2014).