Phytoestrogenic molecule desmethylicaritin suppressed adipogenesis via Wnt/β-catenin signaling pathway

	Phytoestrogenic molecule desmethylicaritin suppressed adipogenesis via Wnt/β-catenin signaling pathway
	Xin-Luan Wang; Nan Wang; Li-Zhen Zheng; Xin-Hui Xie; Dong Yao; Ming-Yan Liu; Zhi-Hong Yao; Yi Dai; Ge Zhang; Xin-Sheng Yao
刊名	EUROPEAN JOURNAL OF PHARMACOLOGY
	2013
英文摘要	Epimedium flavonoids inhibit extravascular lipid deposition during prevention of steroid-associated osteonecrosis. Desmethylicaritin is a bioactive metabolite of Epimedium flavonoids in serum. As it is well known that estrogen inhibits aidpogenesis, so we hypothesized that desmethylicaritin as a phytoestrogen might have the potential to inhibit lipid deposition. This study was designed to investigate the effect of desmethylicaritin on adipogenesis and its underlying mechanism in vitro. Adipogenesis was assessed by Oil Red O staining in 3T3-L1 preadipocytes. Bromodeoxyuridine was used to test the clonal expansion. Further, the mRNA level and protein expression of adipgenic and related factors were detected by qRT-PCR and western blot, respectively. The nuclear location of beta-catenin was identified using immunofluoresence assay. Our results showed that desmethylicaritin suppressed the adipogenesis in 3T3-L1 cells in a dose-dependent manner. In addition, desmethylicaritin inhibited clonal expansion during adipogenesis. Desmethylicaritin did not affect CCAAT/enhancer binding protein delta and beta mRNA expression, but decreased the mRNA expression of CCAAT/enhancer binding protein alpha, peroxisome proliferator-activated receptor gamma, adipocyte lipid-binding protein and lipoprotein lipase. Desmethylicaritin up-regulated the mRNA expression of WntlOb that was however down-regulated after adipogenic induction. Desmethylicaritin increased the protein expression of beta-catenin both in the cytoplasm and nuclei and immunofluorescence results confirmed that desmethylicaritin increased nuclear translocation of beta-catenin. Above findings implied that desmethylicaritin was able to inhibit adipogenesis and Wnt/beta-catenin signaling pathway was regulated by desmethylicaritin in the process of suppression of adipogenesis. Above findings supported desmethylicaritin as a novel phytochemical agent for potential prevention of disorders involving lipid metabolism.
收录类别	SCI
原文出处	http://www.sciencedirect.com/science/article/pii/S0014299913004743
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/4895]
专题	深圳先进技术研究院_医工所
作者单位	EUROPEAN JOURNAL OF PHARMACOLOGY
推荐引用方式 GB/T 7714	Xin-Luan Wang,Nan Wang,Li-Zhen Zheng,et al. Phytoestrogenic molecule desmethylicaritin suppressed adipogenesis via Wnt/β-catenin signaling pathway[J]. EUROPEAN JOURNAL OF PHARMACOLOGY,2013.
APA	Xin-Luan Wang.,Nan Wang.,Li-Zhen Zheng.,Xin-Hui Xie.,Dong Yao.,...&Ling Qin.(2013).Phytoestrogenic molecule desmethylicaritin suppressed adipogenesis via Wnt/β-catenin signaling pathway.EUROPEAN JOURNAL OF PHARMACOLOGY.
MLA	Xin-Luan Wang,et al."Phytoestrogenic molecule desmethylicaritin suppressed adipogenesis via Wnt/β-catenin signaling pathway".EUROPEAN JOURNAL OF PHARMACOLOGY (2013).