Identification, Characterization, and Effects of Xenopus laevis PNAS-4 Gene on Embryonic Development

	Identification, Characterization, and Effects of Xenopus laevis PNAS-4 Gene on Embryonic Development
	Fei Yan; Xu-zhi Ruan; Han-shuo Yang; Shao-hua Yao; Xin-yu Zhao; Lan-tu Gou; Fan-xin Ma; Zhu Yuan; Hong-xin Deng; Yu-quan Wei
刊名	JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY
	2010
英文摘要	Apoptosis plays an important role in embryonic development. PNAS-4 has been demonstrated to induce apoptosis in several cancer cells. In this study, we cloned Xenopus laevis PNAS-4 (xPNAS-4), which is homologous to the human PNAS-4 gene. Bioinformatics analysis for PNAS-4 indicated that xPNAS-4 shared 87.6% identity with human PNAS-4 and 85.5% with mouse PNAS-4. The phylogenetic tree of PNAS-4 protein was also summarized. An analysis of cellular localization using an EGFP-fused protein demonstrated that xPNAS-4 was localized in the perinuclear region of the cytoplasm. RT-PCR analysis revealed that xPNAS-4, as a maternally expressed gene, was present in all stages of early embryo development. Whole-mount in situ hybridization showed that xPNAS-4 was mainly expressed in ectoderm and mesoderm. Furthermore, microinjection of xPNAS-4 mRNA in vivo caused developmental defects manifesting as a small eye phenotype in the Xenopous embryos, and as a small eye or one-eye phenotype in developing zebrafish embryos. In addition, embryos microinjected with xPNAS-4 antisense morpholino oligonucleotides (MOs) exhibited a failure of head development and shortened axis.
收录类别	SCI
原文出处	http://scholar.google.com/scholar_lookup?doi=10%2E1155%2F2010%2F134764&issn=1110-7243&journal=JOURNAL%20OF%20BIOMEDICINE%20AND%20BIOTECHNOLOGY&hl=zh-CN&title=Identification%2C%20Characterization%2C%20and%20Effects%20of%20Xenopus%20laevis%20PNAS-4%20Gene%20on%20Embryonic%20Development&author=F%20Yan&publication_year=2010
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/2929]
专题	深圳先进技术研究院_医工所
作者单位	JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY
推荐引用方式 GB/T 7714	Fei Yan,Xu-zhi Ruan,Han-shuo Yang,et al. Identification, Characterization, and Effects of Xenopus laevis PNAS-4 Gene on Embryonic Development[J]. JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY,2010.
APA	Fei Yan.,Xu-zhi Ruan.,Han-shuo Yang.,Shao-hua Yao.,Xin-yu Zhao.,...&Yu-quan Wei.(2010).Identification, Characterization, and Effects of Xenopus laevis PNAS-4 Gene on Embryonic Development.JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY.
MLA	Fei Yan,et al."Identification, Characterization, and Effects of Xenopus laevis PNAS-4 Gene on Embryonic Development".JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY (2010).