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	An integrated microfluidic digital PCR system for alginate droplet formation efficient direct PCR amplification and imaging
	Lijun Li; Linfen Yu; Tianzhun Wu
	2016
会议名称	MNF2016
会议地点	Milan
英文摘要	Microfluidic based digital PCR (dPCR) enables precise, highly sensitive absolute quantification for nucleic acids without the need for standard curves [1]. For example, by segregating single DNA molecules in individual water-in-oil emulsion droplets, emulsion PCR (ePCR) offers the advantage of massively parallel clonal amplification of DNA templates, which allows the identification and quantization of rare mutant gene within a large population [2, 3] and enables new generation of ultra-high throughput DNA sequencing technologies [4]. More recently, microfluidic microdroplet technology has been exploited to perform PCR in droplets because of its unique features. It can prevent crossover contamination and PCR inhibition, is suitable for single-cell and single-molecule analyses, and has the potential for system integration and automation. Although significant advances have been made in developing droplet-based microfluidic PCR devices, most of them were still cannot integrate droplet formation, PCR amplification, and fluorescent signal detection into one machine. An ideal microfluidic system with implemented on-chip PCR function should be fully autonomous, easy for non-experts to use, and has complete protocol integration. In this paper, we developed a high throughput technology enabling formation and encapsulating PCR sample into solid alginate bead, locating the beads into individual chambers, which allow the on-chip PCR amplification and fluorescent imaging. We demonstrate an integrated and automatic digital PCR system, making it a promising platform for many single copy genetic studies. This platform will also greatly improve our understanding of disease pathophysiology and diagnosis.
收录类别	其他
语种	英语
内容类型	会议论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/10582]
专题	深圳先进技术研究院_医工所
作者单位	2016
推荐引用方式 GB/T 7714	Lijun Li,Linfen Yu,Tianzhun Wu. An integrated microfluidic digital PCR system for alginate droplet formation efficient direct PCR amplification and imaging[C]. 见:MNF2016. Milan.

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