Efficient production of cynomolgus monkeys with a toolbox of enhanced assisted reproductive technologies

	Efficient production of cynomolgus monkeys with a toolbox of enhanced assisted reproductive technologies
	Ma, Yunhan; Li, Jiayu; Wang, Ge; Ke, Qiong; Qiu, Sien; Gao, Liang; Wan, Haifeng; Zhou, Yang; Xiang, Andy Peng; Huang, Qunshan
刊名	SCIENTIFIC REPORTS
	2016
英文摘要	The efficiency of assisted reproductive technologies (ARTs) in nonhuman primates is low due to no screening criterions for selecting sperm, oocyte, and embryo as well as its surrogate mothers. Here we analyzed 15 pairs of pregnant and non-pregnant cynomolgus monkeys, each pair of which received embryos from one batch of fertilized oocytes, and found ratio of endometrial to myometrial thicknesses in abdominal ultrasonic transverse section of uterus is a reliable indicator for selection of recipients for embryo transfer. We performed 305 ovarian stimulations in 128 female cynomolgus monkeys and found that ovarian stimulation can be performed in a whole year and repeated up to six times in the same monkey without deteriorating fertilization potential of eggs until a poor response to stimulation happened. Fertilization can be efficiently achieved with both conventional and piezo-driven intracytoplasmic sperm injection procedures. In semen collection, semen quality is higher with the penile robe electrical stimulus method compared with the rectal probe method. Moreover, caesarean section is an effective strategy for increasing baby survival ratesof multiple pregnancies. These findings provide a practical guidance for the efficient use of ARTs, facilitating their use in genetic engineering of macaque monkeys for basic and translational neuroscience research.
收录类别	SCI
原文出处	http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4865753/
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/9786]
专题	深圳先进技术研究院_脑所
作者单位	SCIENTIFIC REPORTS
推荐引用方式 GB/T 7714	Ma, Yunhan,Li, Jiayu,Wang, Ge,et al. Efficient production of cynomolgus monkeys with a toolbox of enhanced assisted reproductive technologies[J]. SCIENTIFIC REPORTS,2016.
APA	Ma, Yunhan.,Li, Jiayu.,Wang, Ge.,Ke, Qiong.,Qiu, Sien.,...&Yang, Shihua.(2016).Efficient production of cynomolgus monkeys with a toolbox of enhanced assisted reproductive technologies.SCIENTIFIC REPORTS.
MLA	Ma, Yunhan,et al."Efficient production of cynomolgus monkeys with a toolbox of enhanced assisted reproductive technologies".SCIENTIFIC REPORTS (2016).