Platelet-derived growth factor BB gene-released scaffolds: biosynthesis and characterization

CORC > 上海硅酸盐研究所 > 中国科学院上海硅酸盐研究所 > 生物材料与组织工程研究中心 > 期刊论文

	Platelet-derived growth factor BB gene-released scaffolds: biosynthesis and characterization
	Zhang, Yufeng 1,2; Ma, Yihui 1,2; Wu, Chengtie 3; Miron, Richard J.1,2; Cheng, Xiangrong 1,2
刊名	JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
	2016-10-01
卷号	10 期号:10 页码:E372-E381
关键词	self-regeneration PDGF-B adenovirus tissue regeneration scaffold
英文摘要	Tissue engineering generally requires three basic elements; stem/ progenitor cells, inductive agents and a biomaterial scaffold; the latter is one of the key components which directly influences cellular activity and matrix formation. Commonly used scaffolds to repair defects in general do not induce stem cell recruitment, which is an essential element to tissue regeneration. In this study, fabrication of a scaffold which is capable of restoring damaged tissue through the recruitment of mesenchymal stem cells (MSCs) by gene therapy of the gene encoding platelet-derived growth factor-B (PDGF-B) was investigated. PDGF-B adenovirus (AdPDGF) was combined into novel mesoporous bioglass-silk fibrin scaffolds, which were characterized for their controlled release and sustained bioactivity. Our results demonstrate that these scaffolds can release PDGF-B adenovirus for up to 3 weeks and increase MSC recruitment, both in vitro and following subcutaneous implantation in mice. Osseous calvarial defects in mice further demonstrate the ability of these scaffolds to enhance tissue regeneration through stem cell homing. This study demonstrates the potent ability of host stem cells to regenerate tissue defects through recruitment of MSCs via gene therapy. Copyright (C) 2013 John Wiley & Sons, Ltd.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Technology
类目[WOS]	Cell & Tissue Engineering ; Biotechnology & Applied Microbiology ; Cell Biology ; Engineering, Biomedical
研究领域[WOS]	Cell Biology ; Biotechnology & Applied Microbiology ; Engineering
关键词[WOS]	MESENCHYMAL STEM-CELLS ; BONE-MARROW ; PROGENITOR CELLS ; HUMAN BLASTOCYSTS ; EXHIBITS SAFETY ; STROMAL CELLS ; CLINICAL-USE ; PDGF-BB ; ADENOVIRUS ; DELIVERY
收录类别	SCI
语种	英语
WOS记录号	WOS:000387786200015
内容类型	期刊论文
源URL	[http://ir.sic.ac.cn/handle/331005/21948]
专题	上海硅酸盐研究所_生物材料与组织工程研究中心_期刊论文
作者单位	1.Wuhan Univ, Minist Educ Sch & Hosp Stomatol, State Key Lab Breeding Base Basic Sci Stomatol Hu, 237 Luoyu Rd, Wuhan 430079, Peoples R China 2.Wuhan Univ, Minist Educ Sch & Hosp Stomatol, Key Lab Oral Biomed, 237 Luoyu Rd, Wuhan 430079, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Ceram, State Key Lab High Performance Ceram & Superfine, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Yufeng,Ma, Yihui,Wu, Chengtie,et al. Platelet-derived growth factor BB gene-released scaffolds: biosynthesis and characterization[J]. JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE,2016,10(10):E372-E381.
APA	Zhang, Yufeng,Ma, Yihui,Wu, Chengtie,Miron, Richard J.,&Cheng, Xiangrong.(2016).Platelet-derived growth factor BB gene-released scaffolds: biosynthesis and characterization.JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE,10(10),E372-E381.
MLA	Zhang, Yufeng,et al."Platelet-derived growth factor BB gene-released scaffolds: biosynthesis and characterization".JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE 10.10(2016):E372-E381.