Expression of VEGFR-2 on HaCaT cells is regulated by VEGF and plays an active role in mediating VEGF induced effects

CORC > 昆明动物研究所 > 昆明动物研究所 > 动物模型与人类重大疾病机理重点实验室 > 重大疾病机理的遗传学

	Expression of VEGFR-2 on HaCaT cells is regulated by VEGF and plays an active role in mediating VEGF induced effects
	Yang XH 1; Man XY 1; Cai SQ 1; Yao YG 2; Bu ZY 1; Zheng M[*]1
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2006
卷号	349 期号:1 页码:31-38
关键词	p44/42 MAPK cell proliferation cell migration cell adhesion
ISSN号	0006-291X
通讯作者	minz@zju.edu.cn
合作状况	其它
英文摘要	Vascular endothelial growth factor (VEGF) and its receptor VEGFR-2 play important roles in mitogenesis and chemotaxis of endothelial cells. In normal human skin, VEGF is expressed and secreted by epidermal keratinocytes. Emerging data suggest that keratinocyte-derived VEGF targets other cell types besides the dermal endothelial cells. We have recently showed that keratinocytes from human normal skin expressed all five known VEGF receptors and co-receptors (neuropilin 1 and 2). To define the functional significance of VEGFR-2 in epidermis, we examined its role in a keratinocyte cell line, HaCaT cells, in response to VEGF treatment. Expression of VEGFR-2 on HaCaT cells was confirmed at both RNA and protein levels and was regulated by VEGF(165) treatment. Treatment of HaCaT cells with VEGF(165) induced tyrosine-autophosphorylation of VEGFR-2 and phosphorylation of PLC-gamma and p44/42 MAPK in a time-dependent manner. Preincubation with a neutralizing antibody for VEGFR-2 (MAB3571) completely abrogated these phosphorylation effects. Furthermore, VEGF(165) Stimulated proliferation and migration of HaCaT cells, and this effect was significantly blocked by a pretreatment with MAB3571. Neutralizing VEGFR-2 in HaCaT cells increased cell adhesion during culture. Our results suggest that VEGFR-2 expressed on HaCaT cells plays a crucial role in VEGF-mediated regulation of cell activity.
收录类别	SCI
资助信息	This work was supported by grants from the National Natural Science Foundation of China (NSFC) (no. 30471565) and the Natural Science Foundation of Zhejiang Province (no. 399126).
原文出处	200634931.pdf
语种	英语
公开日期	2010-08-24
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/5985]
专题	昆明动物研究所_重大疾病机理的遗传学
作者单位	1.Department of Dermatology, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, China 2.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China
推荐引用方式 GB/T 7714	Yang XH,Man XY,Cai SQ,et al. Expression of VEGFR-2 on HaCaT cells is regulated by VEGF and plays an active role in mediating VEGF induced effects[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2006,349(1):31-38.
APA	Yang XH,Man XY,Cai SQ,Yao YG,Bu ZY,&Zheng M[*].(2006).Expression of VEGFR-2 on HaCaT cells is regulated by VEGF and plays an active role in mediating VEGF induced effects.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,349(1),31-38.
MLA	Yang XH,et al."Expression of VEGFR-2 on HaCaT cells is regulated by VEGF and plays an active role in mediating VEGF induced effects".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 349.1(2006):31-38.