源真核生物贾第虫与原细菌着丝粒/动粒蛋白的免疫印迹检查

CORC > 昆明动物研究所 > 昆明动物研究所 > 其他

	源真核生物贾第虫与原细菌着丝粒/动粒蛋白的免疫印迹检查
	吴传芬 1; 李靖炎[*]2; 代嘉陵 3; 卢思奇 3; 王永潮 1
刊名	动物学研究
	1996
卷号	17 期号:3 页码:323-330
关键词	着丝粒／动粒蛋白的起源和演化贾第虫原细菌免疫印迹
ISSN号	0254-5853
其他题名	The Responses of Archezoa and Archaebacteria to The Antibodies Against Mammalian Centromere/Kinetochore Proteins
中文摘要	以抗人着丝粒蛋白Ｂ的单抗和多抗以及抗ＣＨＯ细胞动粒蛋白的单抗对源真核生物（ａｒｃｈｅｚｏａ）蓝氏贾第虫（Ｇｉａｒｄｉａｌａｍｂｌｉａ）和分别代表原细菌的３个枝的３种原细菌（Ｈａｌｏｂａｃｔｅｒｉｕｍ、Ｔｈｅｒｍｏｐｌａｓｍａ、Ｓｕｌｆｏｓｐｈａｅｒｅｌｌｕｓ）作了免疫电泳检查，并以小眼虫和大肠杆菌作为对照。结果表明，３种原细菌都呈阳性反应；而且贾第虫的反应情况显然比纤毛虫、眼虫、典型涡鞭毛虫、尖尾虫（Ｏｘｙｒｒｈｉｓ）等单细胞后真核生物的更接近于原细菌的情况。这不仅从一个新的方面为真核细胞起源于古代的原细菌的学说提供了新的佐证，而且从着丝粒／动粒蛋白方面证明了源真核生物贾第虫的原始性。本工作还为认识着丝粒蛋白Ｂ和动粒蛋白的起源和演化提供了线索。
英文摘要	To understand the structure and function of the centromere/kinetochore thoroughly,we need information on its origin and evolution,since every living organization has its own origin and evolutionary history.We analyzed the protein components of the centromere/kinetochore in one archezoa,three archeabacteria and one eubacterium by western blotting with the human centromere protein B (CENP-B) monoclonal antibody,mACA-2,which recognizes the amino end region of human CENP-B; CENP-B polyclonal antibody ra-ACA-2; and mAb37A5,a monoclonal antibody against a peptide of the kinetochore in CHO cells.Giardia lamblia was used as a representative of archezoa,which possess no mitochondria and has 70S prokaryotic ribosomes and therefore is considered the most primitive eukaryotic cell.Three archeabacteria,Halobacterium dachaidenesis sp.nov.F3,Sulfosphaerellus thermoacidophilum S-5,and Thermoplasma acidophilum were used as the representatives of each existing group of archeabacteria—methanogenous-halophilic group,sulfur-dependent thermophilic group and thermoplasma group respectively.E.coli was used the representative of eubacteria.On western blots using the CENP-B monoclonal antibody,mACA-2,Giardia lamblia reacted an 80 kD band that is similar to one of the three bands tound in other protists such as Euglena gracilis and Oxyrris marina (Wu et al.,1996c).Three archeabacteria and E.coli gave negative response to mACA-2 antibody.The CENP-B polyclonal antibody,ra-ACA-2,recognized similar 60 kD and 30 kD bands in Giardia lamblia and the three archeabacteria.The other protists tested also exhibited the two bands,and an additional 50 kD band (Wu et al.,1995a-c).The reference E.coli gave 60 kD band and two bands about 80 kD which were different from those of either archeabacteria or giardia.Western blots of G.lamblias extracts using the kinetochore protein monoclonal antibody,mAb37A5,Giardia lamblia gave 45 kD,50 kD and 120 kD bands,of which the 45 kD and 120 kD bands were also found in other tested protists (Wu et al.,1994).Two archeabacteria,Halobacterium and Sulfosphaerellus,produced 40 kD,45 kD,and 50 kD bands.Another archeabacterium Thermoplasma gave a 35 kD band besides those three.Eubacterium E.coli did not give any positive response.From the results,we can suggest a evolutionary line of mAb37A5 antigen from archeabacteria to protists:Thermoplasma,35 kD,40 kD,45 kD and 50 kD;Halobacterium and Sulfospherellus,40 kD,45 kD and 50 kD;Giardia,45 kD,50 kD and 120 kD;Euglena and Oxyrris,45 kD and 120 kD (Wu et al.,1994).Our results support the hypothesis that the eukaryotic cell evoloved from an ancient archeabacterium,and the nucleus of Giardia is indeed one of the most primitive of the eukaryotic nucleus.Therefore it is in the intermediate position between archeabacteria and eukaryotic cells.Our results suggest that although the centromere/kinetochore is a characteristic structure peculiar to eukaryotic cells,its protein molecular ancestors originated in the archeabacterial ancestor of dukaryotic cells.The differentiation of CENP-Bs N-terminal structure most have occurred after the archeabacterial stage.Acknowledegements:We would like to thank Prof.Dr.Dennis G.Searcy of Univ.of Massachusetts,USA,for giving Thermoplasma acidophilum;Prof.Dr.William C.Earnshaw of Johns Hopkins Univ.School of Medicine,USA,for giving mACA-2 and ra-ACA-2 antibodies;Prof.Dr.Ronald Hancock of Laval Univ.,Canada,for giving mAb37A5 antibody;and Prof.Dr.Laurence D.Etkin of the Department of Molecular Genetics,UTMDACC,USA,for the language.improvement of English manuscript.
收录类别	其他
原文出处	1996173323.pdf
语种	中文
公开日期	2010-08-24
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/3441]
专题	昆明动物研究所_其他昆明动物研究所_细胞与分子进化开放实验室
作者单位	1.北京师范大学生物系,北京1000875 2.中国科学院昆明动物研究所细胞与分子进化开放研究实验室,昆明650223 3.首都医科大学寄生虫学教研室,北京1000054
推荐引用方式 GB/T 7714	吴传芬,李靖炎[*],代嘉陵,等. 源真核生物贾第虫与原细菌着丝粒/动粒蛋白的免疫印迹检查[J]. 动物学研究,1996,17(3):323-330.
APA	吴传芬,李靖炎[*],代嘉陵,卢思奇,&王永潮.(1996).源真核生物贾第虫与原细菌着丝粒/动粒蛋白的免疫印迹检查.动物学研究,17(3),323-330.
MLA	吴传芬,et al."源真核生物贾第虫与原细菌着丝粒/动粒蛋白的免疫印迹检查".动物学研究 17.3(1996):323-330.