A mouse line for inducible and reversible silencing of specific neurons

CORC > 昆明动物研究所 > 昆明动物研究所 > 动物模型与人类重大疾病机理重点实验室 > 学习记忆的分子神经机制

	A mouse line for inducible and reversible silencing of specific neurons
	Hu L 1,2,3; Lan W 1,2,3; Guo H 4; Chai GD 5; Huang K 5; Zhang L 1,2; Huang Y 1,2; Chen XF 4; Zhang L 1,2; Song NN 1,2
刊名	MOLECULAR BRAIN
	2014
卷号	7 期号:X 页码:e68
关键词	Neuron silencing Ligand-gated channel Ivermectin Rosa26
通讯作者	lxu@vip.163.com ; dingyuqiang@gmail.com
合作状况	其它
英文摘要	Background: Genetic methods for inducibly and reversibly inhibiting neuronal activity of specific neurons are critical for exploring the functions of neuronal circuits. The engineered human glycine receptor, called ivermectin (IVM)-gated silencing receptor (IVMR), has been shown to possess this ability in vitro. Results: Here we generated a mouse line, in which the IVMR coding sequence was inserted into the ROSA26 locus downstream of a loxP-flanked STOP cassette. Specific Cre-mediated IVMR expression was revealed by mis-expression of Cre in the striatum and by crossing with several Cre lines. Behavioral alteration was observed in Rosa26-IVMR mice with unilateral striatal Cre expression after systemic administration of IVM, and it could be re-initiated when IVM was applied again. A dramatic reduction in neuron firing was recorded in IVM-treated free moving Rosa26-IVMR; Emx1-Cre mice, and neuronal excitability was reduced within minutes as shown by recording in brain slice. Conclusion: This Rosa26-IVMR mouse line provides a powerful tool for exploring selective circuit functions in freely behaving mice.
收录类别	SCI
资助信息	This work was supported by the Key State Research Program from Ministry of Science and Technology of China (2011CB510005, 2012CB966900, 2013CB835103), National Natural Science Foundation of China (81221001, 81200692, 81101026, 31100788, 31271182, 31030034, 91232724), Science and Technology Commission of Shanghai Municipality (12XD1404800), Shanghai Pujiang Program (12PJ1408800), Key Disciplines Group Construction Project of Pudong Health Bureau of Shanghai (PWZxq2014-04) and Sino-UK Higher Education Research Partnership for PhD Studies.
语种	英语
WOS记录号	WOS:000342700900001
公开日期	2014-11-14
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/8164]
专题	昆明动物研究所_学习记忆的分子神经机制昆明动物研究所_动物模型与人类重大疾病机理重点实验室
作者单位	1.Key Laboratory of Arrhythmias, Ministry of Education of China, East Hospital, Tongji University School of Medicine, Shanghai 200120, China 2.Department of Anatomy and Neurobiology, Tongji University School of Medicine, Shanghai 200092, China 3.School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, UK 4.Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Kunming, Yunnan 650223, China 5.Department of Anesthesiology, East Hospital, Tongji University School of Medicine, Shanghai 200120, China 6.Institutes of Brain Science, State Key Laboratory for Medical Neurobiology, Fudan University, Shanghai 200032, China 7.nstitute of Neuroscience, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China.
推荐引用方式 GB/T 7714	Hu L,Lan W,Guo H,et al. A mouse line for inducible and reversible silencing of specific neurons[J]. MOLECULAR BRAIN,2014,7(X):e68.
APA	Hu L.,Lan W.,Guo H.,Chai GD.,Huang K.,...&Ding YQ[*].(2014).A mouse line for inducible and reversible silencing of specific neurons.MOLECULAR BRAIN,7(X),e68.
MLA	Hu L,et al."A mouse line for inducible and reversible silencing of specific neurons".MOLECULAR BRAIN 7.X(2014):e68.