In vitro generation of myofibroblasts-like cells from liver epithelial progenitor cells of rhesus monkey (Macaca mulatta)

	In vitro generation of myofibroblasts-like cells from liver epithelial progenitor cells of rhesus monkey (Macaca mulatta)
	Ji SH 1,3; Wang XH 3,5; Guo XY 3; Zhao B 3,5; Ji WZ 3; Jin LF[*]1,3; Shu JH 2; Sun AJ 4; Si W 3
刊名	IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
	2011
卷号	47 期号:5-6 页码:383-390
关键词	Epithelial-mesenchymal transition Rhesus monkey Hepatic progenitor cells TGF-beta Myofibroblasts-like cells
通讯作者	lifangj@sohu.com
合作状况	其它
英文摘要	The origin of the myofibroblast, the primary effector cell of liver fibrosis, is still elusive. Here, we report that fluorescence-activated cell sorting purified E-cad + rhesus monkey liver epithelial progenitor cells (mLEPCs) may serve as a potential source for liver myofibroblasts. Adult mLEPCs colonies were cultured in medium containing 2 ng/ml transforming growth factor beta (TGF-beta) and 10% fetal bovine serum (FBS) to induce differentiation. Phenotypic changes of cells were analyzed by morphological observation, immunostaining, and reverse transcription-polymerase chain reaction (RT-PCR). After cultured with TGF-beta and FBS, some cells in adult mLEPCs colonies converted to fibroblasts-like cells. Immunostaining showed that fibroblasts-like cells had acquired the expression of mesenchymal cell marker vimentin but lost the expression of epithelial cell marker CK8. Fibroblasts-like cells were maintained in culture for up to 40 passages. RT-PCR analysis revealed that fibroblasts-like cells had acquired the expression of mesenchymal genes (snail, PAI-1, and collagen I) and lost the expression of epithelial specific genes (E-cad, ZO-1, CK18, and occludin). In addition, more than 60% of fibroblasts-like cells expressed myofibroblastic-related proteins such as alpha SMA, vimentin, and N-cad, which were not presented in mLEPCs. Furthermore, increased cell motility was also detected in these fibroblasts-like cells by time-lapse video observation. Our results demonstrate that hepatic epithelial progenitor cells, mLEPCs, transform to myofibroblast-like cells via epithelial-mesenchymal transition. This finding will facilitate understanding of the origin of myofibroblasts in liver fibrosis.
收录类别	SCI
资助信息	This work was supported by research grants from Zhejiang Provincial Natural Science Foundation of China Y2110911, National 863 High Technology Research and Development Program of China 2005AA219010, 2006AA02A101, and 2006AA02A116, Major State Basic Develop- ment Program 2006CB701505 and 2007CB947701, The Chinese Academy of Sciences KSCX1-YWR-47 and KSCX1-05-02, R&D Infrastructure and Facility Development Program of Yunnan Province 2006PT08-2, Social Science and Technology Development Program of Yunnan Province 2007GH, National S&T Major Project 2009ZX09501- 028, National Key Technology R&D Program 2007BAI33B00, and Chinese National Science Foundation 30700159 and 30700425.
语种	英语
内容类型	期刊论文
源URL	[http://159.226.149.26:8080/handle/152453/10421]
专题	昆明动物研究所_生殖与发育生物学昆明动物研究所_中国科学院昆明灵长类研究中心
作者单位	1.College of Life Science of Shaoxing University, 900# Chennan Dadao, Shaoxing, Zhejiang 312000, China 2.School of Life Science of Zhejiang Sci-Tech University, Hangzhou, Zhejiang, China 3.Kunming Primate Research Center, and Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China 4.Shaoxing People’s Hospital and the First Affiliated Hospital of Shaoxing University, Shaoxing, Zhejiang, China 5.Graduate School, Chinese Academy of Sciences, Beijing, China
推荐引用方式 GB/T 7714	Ji SH,Wang XH,Guo XY,et al. In vitro generation of myofibroblasts-like cells from liver epithelial progenitor cells of rhesus monkey (Macaca mulatta)[J]. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL,2011,47(5-6):383-390.
APA	Ji SH.,Wang XH.,Guo XY.,Zhao B.,Ji WZ.,...&Si W.(2011).In vitro generation of myofibroblasts-like cells from liver epithelial progenitor cells of rhesus monkey (Macaca mulatta).IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL,47(5-6),383-390.
MLA	Ji SH,et al."In vitro generation of myofibroblasts-like cells from liver epithelial progenitor cells of rhesus monkey (Macaca mulatta)".IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL 47.5-6(2011):383-390.