Interleukin-2 ((IL-2) gene polymorphism and association with heat tolerance in Nigerian goats

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	Interleukin-2 ((IL-2) gene polymorphism and association with heat tolerance in Nigerian goats
	Yakubu A[]1,2,3; Salako AE 3; Wheto M 7; Okpeku M 8,9; Imumorin IG[]1; Donato MD 1,4; Takeet MI 1,5; Peters SO 6
刊名	SMALL RUMINANT RESEARCH
	2016
卷号	14 期号:X 页码:127-134
关键词	IL-2 Polymorphisms Heat stress Phylogeny Coats
通讯作者	abdulmojyak@gmail.com
英文摘要	Interleukin-2 (IL-2) serves as a potent immuno-modulator, and plays a unique role in both the activation and maintenance of immune responses and in lymphocyte development. Gene polymorphism in IL-2 gene in three major Nigerian goat breeds [West African Dwarf (WAD), Red Sokoto (RS) and Sahel (SH)] was analysed by restriction fragment length polymorphisms (RFLP). The restriction enzyme, Dral was utilized. The association between the polymorphic site and some heat tolerant traits were also investigated in a total of 157 animals comprising 59 WAD, 69 RS, and 29 SH goats of both sexes. The IL-2 sequence displayed a 98% nucleotide identity with the referenced Cahi-IL-2 AY603404.1 orthologous sequence in GenBank. The phylogenetic trees revealed largely species-wise clustering, while the RS and SH goats were closer compared to the WAD goats. DraI genotype frequencies were in Hardy-Weinberg Equilibrium (P<0.05). The expected heterozygosity (H), which is a measure of gene diversity in the goat populations, ranged from 0.26 to 0.28. The heterozygote (TA) had a comparative advantage over the homozygote (AA) in terms of rectal temperature (39.21 +/- 0.08 (TT) and 38.86 +/- 0.13 (TA); P < 0.05). The SH goats also displayed greater resistance to thermal stress compared to the RS and WAD goats. There were varying sex and interaction effects on the thermal indices. The present findings may find application in the selection of superior animals for resistance to heat stress as well as provide insight to questions about evolution, ecology and conservation of Nigeria indigenous goats.
收录类别	SCI
资助信息	We thank the College of Agriculture & Life Sciences and Mario Einaudi Center for International Studies of Cornell University, Ithaca, NY for financial support for this project. Additional sup- port from the Nigerian Government Tertiary Educational Trust Fund fellowship to AY and MIT is gratefully acknowledged.
语种	英语
内容类型	期刊论文
源URL	[http://159.226.149.26:8080/handle/152453/10769]
专题	昆明动物研究所_遗传资源与进化国家重点实验室
作者单位	1.Animal Genetics and Genomics Laboratory, International Programs, College of Agriculture and Life Sciences, Cornell University, Ithaca, NY 14853, USA 2.Department of Animal Science, Nasarawa State University, Lafia, Nigeria 3.Department of Animal Science, University of Ibadan, Ibadan, Nigeria 4.Laboratorio Genetica Molecular, IBB, Universidad de Oriente, Cumana, Venezuela 5.Department of Veterinary Microbiology and Parasitology, University of Agriculture, Abeokuta, Nigeria 6.Department of Animal Science, Berry College, GA 30249, USA 7.Department of Animal Breeding and Genetics, University of Agriculture, Abeokuta, Nigeria 8.Department of Livestock Production, Niger Delta University, Amassoma, Nigeria 9.State Key Laboratory of Genetic Resources and Evolution, Chinese Academy of Science (CAS), Kunming Institute of Zoology, Kunming, Yunnan, China
推荐引用方式 GB/T 7714	Yakubu A[*],Salako AE,Wheto M,et al. Interleukin-2 ((IL-2) gene polymorphism and association with heat tolerance in Nigerian goats[J]. SMALL RUMINANT RESEARCH,2016,14(X):127-134.
APA	Yakubu A[].,Salako AE.,Wheto M.,Okpeku M.,Imumorin IG[].,...&Peters SO.(2016).Interleukin-2 ((IL-2) gene polymorphism and association with heat tolerance in Nigerian goats.SMALL RUMINANT RESEARCH,14(X),127-134.
MLA	Yakubu A[*],et al."Interleukin-2 ((IL-2) gene polymorphism and association with heat tolerance in Nigerian goats".SMALL RUMINANT RESEARCH 14.X(2016):127-134.