蓝氏贾第虫组蛋白的初步研究

	蓝氏贾第虫组蛋白的初步研究
	吴刚 1; 李靖炎 1; 卢思奇 2
刊名	动物学研究
	1996
卷号	17 期号:3 页码:301-305
关键词	蓝氏贾第虫核被膜缺口核被膜的起源
ISSN号	0254-5853
其他题名	Preliminary Study on The Histones of Giardia lamblia
中文摘要	本文初步比较分析了贾第虫组蛋白的存在及其基本组成。通过甲醇固定，两次０．３ｍｏｌ／ＬＨＣｌ抽提及丙酮沉淀，提取贾第虫酸溶性蛋白，利用亲和层析制取总ＤＮＡ结合蛋白，经酸性尿素系统及ＳＤＳ系统电泳分析表明，贾第虫已存在５种组蛋白，其在两种不同性质电泳系统中的电泳行为与相应的小牛胸腺组蛋白有近似的对应关系。说明贾第虫的组蛋白已发生了分化，并已初步形成了性质不同的几个组份，从而在一定程度上支持核小体组蛋白在真核生物的原核祖先阶段就已产生了分化的假说。
英文摘要	About how the prokaryotic ancestor molecular of core histones evolved into four kinds of core histones of eukaryote has two possibilities:one is the ancestral molecular had already differentiated into four kinds at stage of prokaryote,the other is this differentiation occurred after the cell evolving into the eukaryotic cell.HMf and HMt,two kinds of histone-related proteins,isolated respectively from Methanothermus fervidus and Methanobacterium thermoautotrophicum △H,Archaebacteria which are most closely related to eukaryote,by reeves laboratory have already differentiated into HMf1,HMf2 and HMt1,HMt2.Up to now,the core histones found from lower eukaryotic cell,such as Leishmania and Tetrahymena,have all differentiated into four kinds.In this paper,the total histones were prepared from Giardia lamblia,the most primitive eukaryotic cell,by engaging in methanol prefixation,two times of 0.3 mol/L HCl extraction,acetone precipitation and DNA affinity chromatography.The results of electrophoresis,SDS gel and acid urea gel,of the Giardia histones showed that seven major protein bands could be resolved,and five of these bands migrated to position similar those of five kinds of calf thymus histones.It seems,therefore,that the Giardia histones have already differentiated and consisted of at least five kinds of histones,and,on certain extent,this supported the hypothesis that the histone had already differentiated into four different kinds at the stage of prokaryotic ancestor of eukaryote.
收录类别	其他
原文出处	1996173301.pdf
语种	中文
公开日期	2010-08-24
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/3433]
专题	昆明动物研究所_细胞与分子进化开放实验室昆明动物研究所_其他
作者单位	1.中国科学院昆明动物研究所细胞与分子进化开放研究实验室,昆明650223 2.首都医科大学寄生虫学教研室
推荐引用方式 GB/T 7714	吴刚,李靖炎,卢思奇. 蓝氏贾第虫组蛋白的初步研究[J]. 动物学研究,1996,17(3):301-305.
APA	吴刚,李靖炎,&卢思奇.(1996).蓝氏贾第虫组蛋白的初步研究.动物学研究,17(3),301-305.
MLA	吴刚,et al."蓝氏贾第虫组蛋白的初步研究".动物学研究 17.3(1996):301-305.