Outbred genome sequencing and CRISPR/Cas9 gene editing in butterflies

	Outbred genome sequencing and CRISPR/Cas9 gene editing in butterflies
	Li XY 1; Fan DD 2; Zhang W 3; Liu GC 1; Zhang L 2,4; Zhao L 1,5; Fang XD 2; Chen L 1,6; Kronforst MR[]3; Wang W[]1
刊名	NATURE COMMUNICATIONS
	2015
卷号	6 期号:X 页码:e8212
通讯作者	mkronforst@uchicago.edu ; wwang@mail.kiz.ac.cn
合作状况	其它
英文摘要	Butterflies are exceptionally diverse but their potential as an experimental system has been limited by the difficulty of deciphering heterozygous genomes and a lack of genetic manipulation technology. Here we use a hybrid assembly approach to construct high-quality reference genomes for Papilio xuthus (contig and scaffold N50: 492 kb, 3.4 Mb) and Papilio machaon (contig and scaffold N50: 81 kb, 1.15 Mb), highly heterozygous species that differ in host plant affiliations, and adult and larval colour patterns. Integrating comparative genomics and analyses of gene expression yields multiple insights into butterfly evolution, including potential roles of specific genes in recent diversification. To functionally test gene function, we develop an efficient (up to 92.5%) CRISPR/Cas9 gene editing method that yields obvious phenotypes with three genes, Abdominal-B, ebony and frizzled. Our results provide valuable genomic and technological resources for butterflies and unlock their potential as a genetic model system.
收录类别	SCI
资助信息	This project was funded by grants from the National Natural Science Foundation of China (number 31321002), Chinese Academy of Sciences (XDB13000000) and from Yunnan Provincial Science and Technology Department (number 2013HA004) to W.W. M.R.K. and W.Z. were supported by the National Institutes of Health Grant GM108626- 02. We thank D. Yang and Y. Peng for kindly providing their greenhouse; Y. Wu, C. Zhou., Z. Dong and M. Li for help or assistance in butterfly husbandry; Y. Huang for the kind gift of Cas9 expression vector; A. Tan and X. Huang for helpful suggestions in Cas9 experiments; J. Lyu for fruitful discussion in data analysis; and Q. Li for helping in off-target identification.
语种	英语
内容类型	期刊论文
源URL	[http://159.226.149.26:8080/handle/152453/9444]
专题	昆明动物研究所_基因起源组昆明动物研究所_遗传资源与进化国家重点实验室
作者单位	1.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 2.BGI- Shenzhen, Shenzhen 518083, China 3.Department of Ecology and Evolution, University of Chicago, Chicago, Illinois 60637, USA 4.City University of Hongkong, Hongkong, China 5.Department of Evolution and Ecology, University of California Davis, Davis, California 95616, USA 6.University of Chinese Academy of Sciences, Beijing 100049, China 7.School of Bioscience and Biotechnology, South China University of Technology, Guangzhou 510641, China 8.Centre for Social Evolution, Department of Biology, Universitetsparken 15, University of Copenhagen, Copenhagen DK-2100, Denmark
推荐引用方式 GB/T 7714	Li XY,Fan DD,Zhang W,et al. Outbred genome sequencing and CRISPR/Cas9 gene editing in butterflies[J]. NATURE COMMUNICATIONS,2015,6(X):e8212.
APA	Li XY.,Fan DD.,Zhang W.,Liu GC.,Zhang L.,...&Wang W[*].(2015).Outbred genome sequencing and CRISPR/Cas9 gene editing in butterflies.NATURE COMMUNICATIONS,6(X),e8212.
MLA	Li XY,et al."Outbred genome sequencing and CRISPR/Cas9 gene editing in butterflies".NATURE COMMUNICATIONS 6.X(2015):e8212.