A single amino acid defines cross-species reactivity of tree shrew (Tupaia belangeri) CD1d to human invariant natural killer T (iNKT) cells

	A single amino acid defines cross-species reactivity of tree shrew (Tupaia belangeri) CD1d to human invariant natural killer T (iNKT) cells
	Zhang P 1,2; Li DM[]3; Stewart-Jones G 3; Shao XL 1; Zhang YX 2; Chen QY 3; Li YJ 1; He YW 1,4; Xu XN[]1,3; Zhang HT[*]1
刊名	IMMUNOLOGY
	2009
卷号	128 期号:4 页码:500-510
关键词	antigen presentation CD1d antigen T lymphocytes Tupaiidae
ISSN号	0019-2805
通讯作者	demin.li@imm.ox.ac.uk ; xiaoning.xu@imm.ox.ac.uk ; zhanght@post.kiz.ac.cn
合作状况	其它
英文摘要	The non-classical major histocompatibility complex (MHC) class I molecule CD1d presents lipid antigens to invariant natural killer T (iNKT) cells, which are an important part of the innate immune system. CD1d/iNKT systems are highly conserved in evolution, and cross-species reactivity has been suggested to be a common feature of different animals based on research in humans and mice. However, we found that CD1d from the tree shrew (Tupaia belangeri), a close evolutionary relative of primates, failed to stimulate human iNKT cells, despite being more homologous to human CD1d than that of mouse. Sequence comparison and molecular modelling showed that two of the key amino acid residues in human CD1d proposed to be in direct contact with T-cell receptors were mutated in tree shrew CD1d. Substitution of one of the residues, but not the other, with the human residue enabled tree shrew CD1d to regain the ability to present lipid antigen to human iNKT cells. These results indicate that CD1d/iNKT recognition is species-specific, and that cross-species reactivity may be less common than currently proposed. Also, a naturally occurring CD1d mutation(s) that confers inability to stimulate iNKT cell function may have implications for future studies on CD1d/iNKT-associated diseases.
学科主题	Immunology
收录类别	SCI
资助信息	This work was supported by the UK Medical Research Council, Chinese National 973 Program (2007CB512807) and Chinese Academy of Sciences grants for 100’s Talents and Overseas Scholar Team.
语种	英语
公开日期	2010-08-24
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/4433]
专题	昆明动物研究所_分子免疫生物学昆明动物研究所_动物模型与人类重大疾病机理重点实验室
作者单位	1.Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Insti- tute of Zoology, Chinese Academy of Science, Kunming, Yunnan Province, China 2.Graduate University of Chinese Academy of Sciences, Beijing, China 3.MRC Human Immunology Unit, Weatherall Institute of Molecular Medi- cine, John Radcliffe Hospital, Oxford Univer- sity, Oxford, UK 4.Department of Immunology, Duke University Medical Center, Durham, NC, USA
推荐引用方式 GB/T 7714	Zhang P,Li DM[*],Stewart-Jones G,et al. A single amino acid defines cross-species reactivity of tree shrew (Tupaia belangeri) CD1d to human invariant natural killer T (iNKT) cells[J]. IMMUNOLOGY,2009,128(4):500-510.
APA	Zhang P.,Li DM[].,Stewart-Jones G.,Shao XL.,Zhang YX.,...&Zhang HT[].(2009).A single amino acid defines cross-species reactivity of tree shrew (Tupaia belangeri) CD1d to human invariant natural killer T (iNKT) cells.IMMUNOLOGY,128(4),500-510.
MLA	Zhang P,et al."A single amino acid defines cross-species reactivity of tree shrew (Tupaia belangeri) CD1d to human invariant natural killer T (iNKT) cells".IMMUNOLOGY 128.4(2009):500-510.