The sequence and de novo assembly of the giant panda genome

CORC > 昆明动物研究所 > 昆明动物研究所 > 遗传资源与进化国家重点实验室 > 分子进化基因组学

	The sequence and de novo assembly of the giant panda genome
	Li RQ 1,2; Fan W 1; Tian G 1,3; Zhu HM 1; He L 4,5; Cai J 3,6; Huang QF 1; Wang W 6; Zhang YP 6; Wang Jiang[*]1
刊名	NATURE
	2010
卷号	463 期号:7279 页码:311-317
通讯作者	wangjian@genomics.org.cn ; wangj@genomics.org.cn
合作状况	其它
英文摘要	Using next-generation sequencing technology alone, we have successfully generated and assembled a draft sequence of the giant panda genome. The assembled contigs (2.25 gigabases (Gb)) cover approximately 94% of the whole genome, and the remaining gaps (0.05 Gb) seem to contain carnivore-specific repeats and tandem repeats. Comparisons with the dog and human showed that the panda genome has a lower divergence rate. The assessment of panda genes potentially underlying some of its unique traits indicated that its bamboo diet might be more dependent on its gut microbiome than its own genetic composition. We also identified more than 2.7 million heterozygous single nucleotide polymorphisms in the diploid genome. Our data and analyses provide a foundation for promoting mammalian genetic research, and demonstrate the feasibility for using next-generation sequencing technologies for accurate, cost-effective and rapid de novo assembly of large eukaryotic genomes.
收录类别	SCI
资助信息	Thisprojectis supported by the National Natural Science Foundation of China (30725008), Ole Romer grantfromtheDanishNaturalScience Research Council, theSolexaproject (272-07-0196), and Danish Strategic Research Council (2106-07-0021).
语种	英语
内容类型	期刊论文
源URL	[http://159.226.149.26:8080/handle/152453/10464]
专题	昆明动物研究所_分子进化基因组学昆明动物研究所_遗传资源与进化国家重点实验室昆明动物研究所_基因起源组
作者单位	1.BGI-Shenzhen, Shenzhen 518083, China 2.Department of Biology, University of Copenhagen, Copenhagen DK-2200, Denmark 3.The Graduate University of Chinese Academy of Sciences,Beijing100062,China 4.InstitutesofBiomedicalSciences,FudanUniversity,138YixueyuanRoad,Shanghai200032,China 5.Bio-XCenter,KeyLaboratoryfortheGeneticsof Developmental and Neuropsychiatric Disorders (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200030, China 6.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 7.Genome Research Institute, Shenzhen University Medical School, Shenzhen 518000, China 8.Chengdu Research Base of Giant Panda Breeding, Chengdu 610081, China 9.Key Lab of Animal Ecology and Conservation Biology, Institute of Zoology, the Chinese Academy of Sciences, Beichenxilu 1-5, Chaoyang District, Beijing 100101, China. 10.Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK
推荐引用方式 GB/T 7714	Li RQ,Fan W,Tian G,et al. The sequence and de novo assembly of the giant panda genome[J]. NATURE,2010,463(7279):311-317.
APA	Li RQ.,Fan W.,Tian G.,Zhu HM.,He L.,...&Vinar T.(2010).The sequence and de novo assembly of the giant panda genome.NATURE,463(7279),311-317.
MLA	Li RQ,et al."The sequence and de novo assembly of the giant panda genome".NATURE 463.7279(2010):311-317.