Biodiversity soup: metabarcoding of arthropods for rapid biodiversity assessment and biomonitoring

CORC > 昆明动物研究所 > 昆明动物研究所 > 遗传资源与进化国家重点实验室 > 动物生态学研究中心

	Biodiversity soup: metabarcoding of arthropods for rapid biodiversity assessment and biomonitoring
	Yu DW[*]1,2; Ji YQ 1; Ding ZL 3; Emerson BC 2; Wang XY 1; Ye CX 1; Yang CY 1
刊名	METHODS IN ECOLOGY AND EVOLUTION
	2012
卷号	3 期号:4 页码:613-623
关键词	454 Genome Sequencer FLX System DNA barcoding high-throughput sequencing
通讯作者	dougwyu@gmail.com
合作状况	其它
英文摘要	1. Traditional biodiversity assessment is costly in time, money and taxonomic expertise. Moreover, data are frequently collected in ways (e.g. visual bird lists) that are unsuitable for auditing by neutral parties, which is necessary for dispute resolution. 2. We present protocols for the extraction of ecological, taxonomic and phylogenetic information from bulk samples of arthropods. The protocols combine mass trapping of arthropods, mass-PCR amplification of the COI barcode gene, pyrosequencing and bioinformatic analysis, which together we call metabarcoding. 3. We construct seven communities of arthropods (mostly insects) and show that it is possible to recover a substantial proportion of the original taxonomic information. We further demonstrate, for the first time, that metabarcoding allows for the precise estimation of pairwise community dissimilarity (beta diversity) and within-community phylogenetic diversity (alpha diversity), despite the inevitable loss of taxonomic information inherent to metabarcoding. 4. Alpha and beta diversity metrics are the raw materials of ecology and the environmental sciences, facilitating assessment of the state of the environment with a broad and efficient measure of biodiversity.
收录类别	SCI
资助信息	For support, we thank Yunnan Province (20080A001), the Chinese Academy of Sciences (0902281081, KSCX2-YW-Z-1027), the National Natural Science Foundation of China (31170498), and the University of East Anglia.
语种	英语
内容类型	期刊论文
源URL	[http://159.226.149.26:8080/handle/152453/10384]
专题	昆明动物研究所_动物生态学研究中心昆明动物研究所_遗传资源与进化国家重点实验室
作者单位	1.Ecology, Conservation, and Environment Center (ECEC), State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, 32 Jiaochang East Rd., Kunming, Yunnan 650223, China 2.School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich, Norfolk NR47TJ, UK 3.Kunming Biodiversity Large-Apparatus Regional Center, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China
推荐引用方式 GB/T 7714	Yu DW[*],Ji YQ,Ding ZL,et al. Biodiversity soup: metabarcoding of arthropods for rapid biodiversity assessment and biomonitoring[J]. METHODS IN ECOLOGY AND EVOLUTION,2012,3(4):613-623.
APA	Yu DW[*].,Ji YQ.,Ding ZL.,Emerson BC.,Wang XY.,...&Yang CY.(2012).Biodiversity soup: metabarcoding of arthropods for rapid biodiversity assessment and biomonitoring.METHODS IN ECOLOGY AND EVOLUTION,3(4),613-623.
MLA	Yu DW[*],et al."Biodiversity soup: metabarcoding of arthropods for rapid biodiversity assessment and biomonitoring".METHODS IN ECOLOGY AND EVOLUTION 3.4(2012):613-623.