| 题名 | 树鼩转录组及天然免疫NOD-like 受体家族基因分析 |
| 作者 | 王光印 |
| 学位类别 | 硕士 |
| 答辩日期 | 2012-05 |
| 授予单位 | 中国科学院研究生院 |
| 授予地点 | 北京 |
| 导师 | 张云 |
| 关键词 | 树鼩 RNA-seq 转录组 NOD-like 基因进化 基因扩增 |
| 其他题名 | The transcriptome and Nod-like receptor family gene analyses of the tree shrew |
| 学位专业 | 生物化学与分子生物学 |
| 中文摘要 | 由于独特的进化地位及解剖学、生理学特性，树鼩作为一种新兴的实验动物， 已经被作为人类疾病动物模型被广泛应用于肿瘤学、免疫学、病毒学以及神经科 学等诸多领域。作为一种成熟的实验动物，必须具有完整的遗传学背景以满足科 学研究对其日益迫切需求。因此，清晰的遗传学背景对加速树鼩开发用于疾病动 物模型研究有重要现实意义。随着测序技术日新月异的发展，RNA-seq 作为新一 代转录组测序技术已经被大家所熟知。本文就开展树鼩转录组的 RNA-seq 开展 了一些研究工作，并且成功提取到树鼩的 7 个组织（肝脏、肾脏、胰脏、脑组织、 心脏、睾丸和卵巢）的高质量 RNA 样本，并送交至华大基因研究院进行测序。 并对所得的转录组数据库做了一些简要的分析。 天然免疫（innate immunity）是多细胞动物最古老的防御外来微生物入侵的 防线之一。作为模式识别受体（PRRs）的成员，NOD-like 受体家族(NOD-like receptors，NLRs)在天然免疫发生以及介导相关特异性免疫中起着至关重要的作 用。NLRs 中大多数成员的配体结合域类似于 TLR，富含亮氨酸。由于 NLRs 成 员与人类多种疾病相关，故极有可能成为构建人类疾病模型的代表实验动物树鼩 的 NLRs 情况也就显得格外重要。本文在华大基因研究院测定的初步注释的树鼩 转录组数据的基础上，进一步利用 MEGA5、Neighbor-joining 和 MrBayes 等软件， 对 NLRs 基因家族以及他们在人、猕猴和小鼠生物体内的进化进行了分析和总 结。然后根据得到的转录组数据中的序列信息，并结合其他物种的 NLRs 全长序 列信息，扩增到了部分 NLRs 基因序列。这为注释树鼩基因组以及 NLRs 家族的 研究积累了的经验，具有一定的理论意义和应用价值。 |
| 英文摘要 | Due to the unique evolutionary position and anatomy physiology, Tree shrews as new experimental animal have being widely used as animal models of human disease in areas of oncology, immunology, virology, and neuroscience. As an experimental animal, there must be the complete genetic background for meeting the increasingly urgent scientific research. Therefore, a clear genetic background for accelerating the development of tree shrews models has important practical significance. With the rapid development of sequencing technology, RNA-seq as a new generation of transcriptome sequencing technology has been known. This paper has carried out discussions on tree shrew RNA-seq, and successfully extracted high-quality RNAs from the seven organizations of tree shrew (liver, kidney, pancreas, brain tissue, heart, testis and ovary), then they were sent to the BGI-Shenzhen sequencing. And there is some brief analysis on the Tree shrew’s transcriptome database. Innate immunity is one of the oldest against microbial invasion protections in multi-cellular animals. As a big family of the pattern recognition receptors (PRRs), NOD-like receptor family (NLRs) plays a vital role in the innate immunity and mediating the specific immune. The ligand binding domains of majority of NLRs members are similar to the TLR, rich in leucine. For the NLRs members are related to many human diseases, it is particularly important to make clear about the NLRs situation of the tree shrew which is most likely to be representative of experimental animal to build animal models of human disease. The NLRs genes of tree shrew from the annotated transcriptome data sent back from BGI-Shenzhen and the ones in human, macaca and mouse have been evolutionary analyzed and summarized by the softwares MEGA5, Neighbor-joining and MrBayes. Partial NLRs gene sequences of tree shrew were amplified. There are some experiences accumulated for the future study of NLRs in tree shrew and the deeper annotation for the tree shrew genome that has a certain theoretical significance and application value. |
| 语种 | 中文 |
| 公开日期 | 2012-09-25 |
| 内容类型 | 学位论文 |
| 源URL | [http://159.226.149.42:8088/handle/152453/7120]  |
| 专题 | 昆明动物研究所_动物活性蛋白多肽组学 |
| 推荐引用方式 GB/T 7714 | 王光印. 树鼩转录组及天然免疫NOD-like 受体家族基因分析[D]. 北京. 中国科学院研究生院. 2012. |
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