Protease-activated receptor (PAR)1, PAR2 and PAR4 expressions in esophageal squamous cell carcinoma

CORC > 昆明动物研究所 > 昆明动物研究所 > 动物模型与人类重大疾病机理重点实验室 > 动物活性蛋白多肽组学

	Protease-activated receptor (PAR)1, PAR2 and PAR4 expressions in esophageal squamous cell carcinoma
	Li SM 1; Jiang P 1; Xiang Y 2; Wang WW 3; Zhu YC 1; Feng WY 1; Li SD 1; Yu GY[*]1
刊名	Zoological research
	2014
卷号	35 期号:5 页码:420−425
关键词	Esophageal squamous cell carcinoma Methylation PAR1 PAR2 PAR4 PCR Western Blot
通讯作者	yuguoyu2011@hotmail.com
合作状况	其它
英文摘要	Here, we used reverse transcription-PCR (RT-PCR) and western blot to detect protease-activated receptor (PAR) 1, PAR 2 and PAR 4 expression in cancer tissues and cell lines of esophageal squamous cell carcinoma, and investigated the co-relationship between PAR expression and clinic-pathological data for esophageal cancer. The methylation of PAR4 gene promoter involved in esophageal carcinoma was also analyzed. By comparing the mRNA expressions of normal esophageal tissue and human esophageal epithelial cells (HEEpiC), we found that among the 28 cases of esophageal squamous cell carcinoma, PAR1 (60%) and PAR2 (71%) were elevated in 17 and 20 cases, respectively, and PAR4 (68%) expression was lowered in 19 cases. Whereas, in human esophageal squamous cells (TE-1 and TE-10), PAR1 and PAR2 expression was increased but PAR4 was decreased. Combined with clinical data, the expression of PAR1 in poorly differentiated (P=0.016) and middle and lower parts of the esophagus (P=0.016) was higher; expression of PAR4 in poorly differentiated carcinoma was lower (P=0.049). Regarding TE-1 and TE-10 protein expression, we found that in randomized esophageal carcinoma, PAR1 (P=0.027) and PAR2 (P=0.039) expressions were increased, but lowered for PAR4 (P=0.0001). In HEEpiC, TE-1, TE-10, esophageal and normal esophagus tissue samples (case No. 7), the frequency of methylation at the 19 CpG loci of PAR4 was 35.4%, 95.2%, 83.8%, 62.6% and 48.2%, respectively. Our results indicate that the expression of PAR1 and PAR2 in esophageal squamous cell carcinoma is increased but PAR4 is decreased. Hypermethylation of the promoter of the PAR4 gene may contribute to reduced expression of PAR4 in esophageal squamous cell carcinoma.
收录类别	其他
资助信息	This study was supported by the National Natural Foundation of China (81160302)，the Major Research Project of Yunnan Province (2011FZ109)，and Research project of Yunnan Education Bureau (2014Y153)
语种	英语
公开日期	2015-06-10
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/8359]
专题	昆明动物研究所_动物活性蛋白多肽组学昆明动物研究所_动物模型与人类重大疾病机理重点实验室
作者单位	1.Kunming Medical University, Kunming 650500, China 2.Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming 650223, China 3.Third Affiliated Hospital of Kunming Medical University, Department of Thoracic Surgery, Kunming 650000, China
推荐引用方式 GB/T 7714	Li SM,Jiang P,Xiang Y,et al. Protease-activated receptor (PAR)1, PAR2 and PAR4 expressions in esophageal squamous cell carcinoma[J]. Zoological research,2014,35(5):420−425.
APA	Li SM.,Jiang P.,Xiang Y.,Wang WW.,Zhu YC.,...&Yu GY[*].(2014).Protease-activated receptor (PAR)1, PAR2 and PAR4 expressions in esophageal squamous cell carcinoma.Zoological research,35(5),420−425.
MLA	Li SM,et al."Protease-activated receptor (PAR)1, PAR2 and PAR4 expressions in esophageal squamous cell carcinoma".Zoological research 35.5(2014):420−425.