Two new types of allergens from the cockroach, Periplaneta americana

CORC > 昆明动物研究所 > 昆明动物研究所 > 动物模型与人类重大疾病机理重点实验室 > 动物毒素室

	Two new types of allergens from the cockroach, Periplaneta americana
	Fang Y 1; Long C 1; Bai X 1; Liu W 1; Rong M 1; Lai R[]1,2,3; An S[]1,4
刊名	ALLERGY
	2015
卷号	70 期号:12 页码:1674-1678
关键词	alpha-amylase anaphylaxis basophil chitinase cockroach allergens
通讯作者	rlai@mail.kiz.ac.cn ; aslxj@mail.ustc.edu.cn
合作状况	其它
英文摘要	Periplaneta americana cockroach is an important source of inhalant indoor allergen resource, there are more than twenty IgE-binding components identified in P. americana, but only 9 allergens were characterized. Our knowledge about cockroach allergens remains poor. In this work, two novel allergen proteins Per a 11 (alpha-amylase) and Per a 12 (chitinase) with molecular weight around 55 and 45 kDa, respectively, were purified and characterized from the midgut of cockroaches. Their primary sequences were determined by Edman degradation, mass spectrometry and cDNA cloning. Sera from 39 and 30 of 47 (83.0% and 63.8%) patients reacted to Per a 11 and Per a 12 on immunoblots, respectively. The allergenicity of Per a 11 and Per a 12 was further confirmed by competitive ELISA, basophil activation test (BAT) and skin prick test (SPT). They appear to be of importance for the allergic reactions induced by cockroach, and have a potential for component-based diagnosis of allergy.
收录类别	SCI
资助信息	This work was supported by MOST (2013CB911300, 2010CB529800), NSFC (31025025, U1132601, 31200590, 81460253), CAS (SAJC201308), and Yunnan Pro- vince (2011CI139, 2012BC009).
语种	英语
内容类型	期刊论文
源URL	[http://159.226.149.26:8080/handle/152453/9436]
专题	昆明动物研究所_动物毒素室昆明动物研究所_动物模型与人类重大疾病机理重点实验室
作者单位	1.Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming, Yunnan 2.Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu 3.Joint Laboratory of Natural peptide, Chinese Academy of Sciences, University of Science and Technology of China and Kunming Institute of Zoology 4.Faculty of Life Science and Technology, Kunming University of Science and Technology, Yunnan, China
推荐引用方式 GB/T 7714	Fang Y,Long C,Bai X,et al. Two new types of allergens from the cockroach, Periplaneta americana[J]. ALLERGY,2015,70(12):1674-1678.
APA	Fang Y.,Long C.,Bai X.,Liu W.,Rong M.,...&An S[*].(2015).Two new types of allergens from the cockroach, Periplaneta americana.ALLERGY,70(12),1674-1678.
MLA	Fang Y,et al."Two new types of allergens from the cockroach, Periplaneta americana".ALLERGY 70.12(2015):1674-1678.