A novel myotropic peptide from the skin secretions of the tree frog, Polypedates pingbianensis

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	A novel myotropic peptide from the skin secretions of the tree frog, Polypedates pingbianensis
	Yan HL 1; Wei L 1; He XQ 1; Liu H 2; Yang SL 2; Lai R[]1,2; Rao DQ[]2
刊名	BIOCHIMIE
	2012
卷号	94 期号:8 页码:1718-1723
关键词	Amphibian Skin Defensive Myotropic Polypedates pingbianensis
通讯作者	rlai72@njau.edu.cn ; raodq@mail.kiz.ac.cn
合作状况	其它
英文摘要	A novel myotropic peptide, polypedatein, was purified and characterized from the skin secretions of the tree frog, Polypedates pingbianensis. Its primary structure, TLLCKYFAIC, was determined by Edman degradation and mass spectrometry. Polypedatein was subjected to bioassays including myotropic, antimicrobial, and serine protease inhibitory activities, which are related with many amphibian skin bioactive peptides. It was found to elicit concentration-dependent contractile effects on isolated rat ileum. cDNA clones encoding the precursor of polypedatein were isolated by screening a skin cDNA library of P. pingbianensis and then sequenced. The amino acid sequence deduced from the cDNA sequences matches well with the result from Edman degradation. BLAST search revealed that the sequence of polypedatein did not show similarity to known protein or peptide sequences. Especially, polypedatein does not contain conserved structural motifs of other amphibian myotropic peptides, such as bradykinins, bombesins, cholecystokinin (CCK), and tachykinins, indicating that polypedatein belongs to a novel amphibian myotropic peptide family. The signal peptide of the precursor encoding polypedatein shows significant sequence identity to that of other amphibian skin defensive peptides, such as antimicrobial peptides, bradykinins, lectins, and serine protease inhibitors, suggesting that polypedatein belongs to a novel amphibian myotropic peptide family. Polypedatein is also the first bioactive peptide from the genus of the frog, Polypedates.
收录类别	SCI
资助信息	This work was supported by Chinese National Natural Science Foundation (30830021, 31025025, 31070701, 31000960, 31025025, U1132601),theMinistryofScienceandTechnology(2010CB529800, 2009ZX09103-1/091, 2011ZX09102-002-10), the Ministry of Agri- culture (2009ZX08009-159B) and Yunnan Province (Y103951111).
语种	英语
WOS记录号	WOS:000306209000010
公开日期	2012-09-13
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/7068]
专题	昆明动物研究所_兽类生态与进化昆明动物研究所_动物模型与人类重大疾病机理重点实验室昆明动物研究所_动物毒素室
作者单位	1.Life Sciences College of Nanjing Agricultural University, 1st Weigang, Nanjing, Jiangsu 210095, China 2.Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Provinces, Kunming Institute of Zoology, Kunming 650223, Yunnan, China
推荐引用方式 GB/T 7714	Yan HL,Wei L,He XQ,et al. A novel myotropic peptide from the skin secretions of the tree frog, Polypedates pingbianensis[J]. BIOCHIMIE,2012,94(8):1718-1723.
APA	Yan HL.,Wei L.,He XQ.,Liu H.,Yang SL.,...&Rao DQ[*].(2012).A novel myotropic peptide from the skin secretions of the tree frog, Polypedates pingbianensis.BIOCHIMIE,94(8),1718-1723.
MLA	Yan HL,et al."A novel myotropic peptide from the skin secretions of the tree frog, Polypedates pingbianensis".BIOCHIMIE 94.8(2012):1718-1723.