Interferon-Lambda3 (IFN-lambda 3) and Its Cognate Receptor Subunits in Tree Shrews (Tupaia belangeri): Genomic Sequence Retrieval, Molecular Identification and Expression Analysis

	Interferon-Lambda3 (IFN-lambda 3) and Its Cognate Receptor Subunits in Tree Shrews (Tupaia belangeri): Genomic Sequence Retrieval, Molecular Identification and Expression Analysis
	Li ML 1,3; Xu WW 1,3; Gao YD 4; Guo Y 1; Wang WJ 1; Wang C 1; Jiang SY 3,4; Willden A 5; Huang JF 4; Zhang HT[*]1,2
刊名	PLOS ONE
	2013
卷号	8 期号:3 页码:e60048
通讯作者	zhanght@mail.kiz.ac.cn
英文摘要	Type III IFNs (IFN-lambda s) constitute a new subfamily with antiviral activities by signaling through a unique receptor complex composed of IFN-lambda s receptor 1 (IFN lambda R1) and interleukin-10 receptor 2 (IL10R2). As tree shrews (Tupaia belangeri) have shown susceptiblility to several human viruses, they are a potentially important model for analyzing viral infection. However, little is known about their IFN-lambda s system. We used the tree shrew genome to retrieve IFN-lambda s and their receptor contig sequences by BLASTN and BLASTZ algorithms, and GenScan was used to scan transcripts from the putative contig sequences. RT-PCR and bioinformatic methods were then used to clone and characterize the IFN-lambda s system. Due to its highest identity with human IFN-lambda 3, we opted to define one intact IFN-lambda gene, tsIFN-lambda 3, as well as its two receptor subunits, tsIFN lambda R1 and tsIL10R2. Additionally, our results showed that tsIFN-lambda 3 contained many features conserved in IFN-lambda 3 genes from other mammals, including conserved signal peptide cleavage and glycosylation sites, and several residues responsible for binding to the type III IFNR. We also found six transcript variants in the receptors: three in tsIFN lambda R1, wherein different extracellular regions exist in three transmembrane proteins, resulting in different affinities with IFN-lambda s; and three more variants in tsIL10R2, encoding one transmembrane and two soluble proteins. Based on tissue distribution in the liver, heart, brain, lung, intestine, kidney, spleen, and stomach, we found that IFN-lambda s receptor complex was expressed in a variety of organs although the expression level differed markedly between them. As the first study to find transcript variants in IL-10R2, our study offers novel insights that may have important implications for the role of IFN-lambda s in tree shrews' susceptibility with a variety of human viruses, bolstering the arguments for using tree shrews as an animal model in the study of human viral infections.
收录类别	SCI
资助信息	This work was supported by grants from the Chinese Academy of Sciences (KSCX2-EW-J-23 and KSCX2-EW-R-12), the National Key Basic Research and Development Program (973 programme, 2007CB512807), the Talents Program of the Chinese Academy of Sciences (No.A0820) and the Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences.
语种	英语
WOS记录号	WOS:000317262200073
公开日期	2013-05-17
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/7427]
专题	昆明动物研究所_分子免疫生物学昆明动物研究所_动物模型与人类重大疾病机理重点实验室昆明动物研究所_遗传资源与进化国家重点实验室昆明动物研究所_结构生物信息学管理、支撑部门_大型仪器中心
作者单位	1.Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming, China 2.Chongqing Center for Biomedical Research and Equipment Development, Chongqing Academy of Science and Technology, Chongqing, China 3.University of the Chinese Academy of Sciences, Beijing, China 4.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China 5.Editorial Department, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China
推荐引用方式 GB/T 7714	Li ML,Xu WW,Gao YD,et al. Interferon-Lambda3 (IFN-lambda 3) and Its Cognate Receptor Subunits in Tree Shrews (Tupaia belangeri): Genomic Sequence Retrieval, Molecular Identification and Expression Analysis[J]. PLOS ONE,2013,8(3):e60048.
APA	Li ML.,Xu WW.,Gao YD.,Guo Y.,Wang WJ.,...&Zhang HT[*].(2013).Interferon-Lambda3 (IFN-lambda 3) and Its Cognate Receptor Subunits in Tree Shrews (Tupaia belangeri): Genomic Sequence Retrieval, Molecular Identification and Expression Analysis.PLOS ONE,8(3),e60048.
MLA	Li ML,et al."Interferon-Lambda3 (IFN-lambda 3) and Its Cognate Receptor Subunits in Tree Shrews (Tupaia belangeri): Genomic Sequence Retrieval, Molecular Identification and Expression Analysis".PLOS ONE 8.3(2013):e60048.