Maternal-effect Floped gene is essential for the derivation of  embryonic stem cells in mice

CORC > 昆明动物研究所 > 昆明动物研究所 > 遗传资源与进化国家重点实验室 > 哺乳动物胚胎发育

	Maternal-effect Floped gene is essential for the derivation of embryonic stem cells in mice
	Zhao B[*]1,2,3; Cun YX 1,2,3; He XC 1,3; Zheng P 1
刊名	Zoological research
	2013
卷号	34 期号:E3 页码:E82−E86
关键词	Floped ICM ESCs
通讯作者	ytuzhaobo@163.com
合作状况	其它
英文摘要	Floped (official name Ooep) is specifically and abundantly expressed in mouse oocytes and embryonic stem cells (ESCs). Depletion of Floped from oocytes leads to early embryonic arrest at the 2-cell stage. Although crucial in cleavage stage development, its roles in early embryos as well as in ESCs remain completely unknown. Here, we compared the efficiency of mouse ESC derivation from inner cell mass (ICM) with and without Floped to study its possible roles in mESCs. Derivation rates of mESC from wild-type, heterozygous, and homozygous blastocysts were 33.3%, 21.43%, and 3.85%, respectively, indicating that Floped-/- blastocysts had significantly decreased derivation rates. Respective outgrowth appearing rate five days after blastocyst attachment were 83.3%, 85.7%, and 15.4%. Morphologically, the outgrowth of ICM from Floped-/- blastocysts appeared severely death three to five days after blastocyst attachment, and the respective derived stem cells showed long-term instability with long-standing epithelial-like colonies. This result suggests a possible role of Floped in the course of ICM-ESCs transition.
收录类别	其他
资助信息	This work was supported by the Chinese NSFC grant (31171424)
语种	英语
公开日期	2013-06-14
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/7467]
专题	昆明动物研究所_哺乳动物胚胎发育昆明动物研究所_遗传资源与进化国家重点实验室
作者单位	1.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 2.University of Chinese Academy of Sciences, Beijing 100049, China 3.Yunnan Key Laboratory of Animal Reproduction Biology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China
推荐引用方式 GB/T 7714	Zhao B[*],Cun YX,He XC,et al. Maternal-effect Floped gene is essential for the derivation of embryonic stem cells in mice[J]. Zoological research,2013,34(E3):E82−E86.
APA	Zhao B[*],Cun YX,He XC,&Zheng P.(2013).Maternal-effect Floped gene is essential for the derivation of embryonic stem cells in mice.Zoological research,34(E3),E82−E86.
MLA	Zhao B[*],et al."Maternal-effect Floped gene is essential for the derivation of embryonic stem cells in mice".Zoological research 34.E3(2013):E82−E86.