A fluorometric assay for alkaline phosphatase activity based on beta-cyclodextrin-modified carbon quantum dots through host-guest recognition

	A fluorometric assay for alkaline phosphatase activity based on beta-cyclodextrin-modified carbon quantum dots through host-guest recognition
	Tang, Cong 1; Qian, Zhaosheng 1; Huang, Yuanyuan 1; Xu, Jiamin 1; Ao, Hang 1; Zhao, Meizhi 1; Zhou, Jin 2; Chen, Jianrong 1; Feng, Hui 1
刊名	BIOSENSORS & BIOELECTRONICS
	2016-09-15
卷号	83 页码:274-280
关键词	Carbon quantum dots Alkaline phosphatase (ALP) activity Real-time fluorometric assay Photoinduced electron transfer (PET)
英文摘要	A convenient, reliable and highly sensitive assay for alkaline phosphatase (ALP) activity in the real-time manner is developed based on beta-cyclodextrin-modified carbon quantum dots (beta-CD-CQDs) nanoprobe through specific host-guest recognition. Carbon quantum dots were first functionalized with 3-amino phenyl boronic acid to produce boronic acid-functionalized CQDs, and then further modified with hydropropyl beta-cyclodextrins (beta-CD) through B-O bonds to form beta-CD-CQDs nanoprobe. p-Nitrophenol phosphate disodium salt is used as the substrate of ALP, and can hydrolyze to p-nitrophenol under the catalysis of ALP. The resulting p-nitrophenol can enter the cavity of beta-CD moiety in the nanoprobe due to their specific host-guest recognition, where photoinduced electron transfer process between p-nitrophenol and CQDs takes place to efficiently quench the fluorescence of the probe. The correlation between quenched fluorescence and ALP level can be used to establish quantitative evaluation of ALP activity in a broad range from 3.4 to 100.0 U/L with the detection limit of 0.9 U/L. This assay shows a high sensitivity to ALP even in the presence of a very high concentration of glucose. This study demonstrates a good electron donor/acceptor pair, which can be used to design general detection strategy through PET process, and also broadens the application of host-guest recognition for enzymes detection in clinical practice. (C) 2016 Elsevier B.V. All rights reserved.
收录类别	SCI
语种	英语
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/35761]
专题	化学研究所_活体分析化学实验室
作者单位	1.Zhejiang Normal Univ, Coll Chem & Life Sci, Jinhua 321004, Peoples R China 2.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Tang, Cong,Qian, Zhaosheng,Huang, Yuanyuan,et al. A fluorometric assay for alkaline phosphatase activity based on beta-cyclodextrin-modified carbon quantum dots through host-guest recognition[J]. BIOSENSORS & BIOELECTRONICS,2016,83:274-280.
APA	Tang, Cong.,Qian, Zhaosheng.,Huang, Yuanyuan.,Xu, Jiamin.,Ao, Hang.,...&Feng, Hui.(2016).A fluorometric assay for alkaline phosphatase activity based on beta-cyclodextrin-modified carbon quantum dots through host-guest recognition.BIOSENSORS & BIOELECTRONICS,83,274-280.
MLA	Tang, Cong,et al."A fluorometric assay for alkaline phosphatase activity based on beta-cyclodextrin-modified carbon quantum dots through host-guest recognition".BIOSENSORS & BIOELECTRONICS 83(2016):274-280.