Conjugation with an Inulin-Chitosan Adjuvant Markedly Improves the Immunogenicity of Mycobacterium tuberculosis CFP10-TB10.4 Fusion Protein | |
Yu, Weili1,2; He, Tao1 | |
刊名 | MOLECULAR PHARMACEUTICS |
2016-11-01 | |
卷号 | 13期号:11页码:3626-3635 |
关键词 | Mycobacterium tuberculosis inulin chitosan adjuvant delivery system subunit vaccine |
ISSN号 | 1543-8384 |
英文摘要 | Protein-based vaccines are of potential to deal with the severe situations posed by Mycobacterium tuberculosis (Mtb). Due to inherently poor immunogenicity of Mtb protein antigens, a potent immunostimulatory adjuvant is needed to enhance the cellular and humoral immune response to Mtb protein antigens. Inulin and chitosan (Cs) are polysaccharide adjuvants that can be used to achieve such an objective. The inulin-Cs conjugate (inulin-Cs) acted as a potent adjuvant through a synergistic interaction of inulin and Cs. CFP10 and TB10.4 are two important virulent protein antigens of Mtb. The CFP10-TB10.4 fusion protein (CT) was constructed and used as the protein antigen. In the present study, an adjuvant delivery system (inulin-Cs-CT) was developed by covalent conjugation of CT with inulin-Cs. Conjugation with inulin-Cs significantly increased the hydrodynamic volume of CT and did not alter the structure of CT. High levels of Thl-type cytokines (IFN-gamma, TNF-alpha, and IL-2) and Th2-type cytokine (IL-4) were secreted by provocation of inulin-Cs-CT. Inulin Cs CT elicited high CT-specific antibody titers, mostly in the form of IgG1 and IgG2b. Pharmacokinetics revealed that conjugation with inulin-Cs could prolong the serum exposure of CT to the immune system. Pharmacodynamics suggested that conjugation with inulin-Cs led to an efficient production of CT-specific IgG. Thus, conjugation of inulin-Cs can serve as a potent adjuvant delivery system to improve the immunogenicity of the Mtb protein antigens. |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
类目[WOS] | Medicine, Research & Experimental ; Pharmacology & Pharmacy |
研究领域[WOS] | Research & Experimental Medicine ; Pharmacology & Pharmacy |
关键词[WOS] | IMMUNE-RESPONSES ; EFFICIENT PROTECTION ; VACCINE ; ANTIGEN ; EFFICACY ; CELL ; NANOPARTICLES ; COMPONENTS ; SYSTEM ; MODEL |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000387428300003 |
内容类型 | 期刊论文 |
源URL | [http://ir.ipe.ac.cn/handle/122111/21652] |
专题 | 过程工程研究所_生化工程国家重点实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, 1 Bei Er Tiao St, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100190, Peoples R China |
推荐引用方式 GB/T 7714 | Yu, Weili,He, Tao. Conjugation with an Inulin-Chitosan Adjuvant Markedly Improves the Immunogenicity of Mycobacterium tuberculosis CFP10-TB10.4 Fusion Protein[J]. MOLECULAR PHARMACEUTICS,2016,13(11):3626-3635. |
APA | Yu, Weili,&He, Tao.(2016).Conjugation with an Inulin-Chitosan Adjuvant Markedly Improves the Immunogenicity of Mycobacterium tuberculosis CFP10-TB10.4 Fusion Protein.MOLECULAR PHARMACEUTICS,13(11),3626-3635. |
MLA | Yu, Weili,et al."Conjugation with an Inulin-Chitosan Adjuvant Markedly Improves the Immunogenicity of Mycobacterium tuberculosis CFP10-TB10.4 Fusion Protein".MOLECULAR PHARMACEUTICS 13.11(2016):3626-3635. |
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