Global DNA methylation and related mRNA profiles in sheep oocytes and early embryos derived from pre-pubertal and adult donors

CORC > 东北地理与农业生态研究所 > 湿地生态系统管理学科组 > 期刊论文

	Global DNA methylation and related mRNA profiles in sheep oocytes and early embryos derived from pre-pubertal and adult donors
	Y. Fang, X. S. Zhang, J. L. Zhang, R. Z. Zhong and D. W. Zhou
刊名	Animal Reproduction Science
	2016
卷号	164 页码:144-151
通讯作者	周道玮
中文摘要	The developmental capacity of in vitro-matured oocytes and in vitro-fertilized embryos from pre-pubertal sheep is less than that of adult counterparts, and epigenetic mechanisms are thought to be involved. In the present study, germinal vesicle stage oocytes were collected by follicular aspiration from superovulated 4-week-old lambs and 2.5-year-old ewes. There were evaluations of the developmental potential of oocytes and embryos by in vitro culture and fertilization, global DNA methylation and hydroxymethylation patterns by immunofluorescence staining, and relative abundance of enzyme mRNA by quantitative real-time polymerase chain reaction analysis in pre-pubertal and adult sheep donors. The results showed that the rates of maturation and cleavage of oocytes as well as pregnancy and lambing rates from the transfer of 2-cell embryos collected from lambs were less than those from adults (P < 0.05). The global DNA methylation and hydroxymethylation and relative abundance of Dnmt1, Dnmt3a, and Teti mRNA were less at all stages of oocytes, zygotes, and two-cell embryos from lambs compared with those from adults (P< 0.05) with no difference in relative abundance of Dnmt3b mRNA. Thus, younger donor age was associated with disturbed DNA methylation processes due to insufficient methyltransferases during gametogenesis and early embryonic development, and this may be responsible for the lesser developmental potential of oocytes and early developing embryos when oocytes are collected from lambs. (C) 2015 Published by Elsevier B.V.
内容类型	期刊论文
源URL	[http://159.226.123.10/handle/131322/7033]
专题	东北地理与农业生态研究所_湿地生态系统管理学科组_期刊论文
推荐引用方式 GB/T 7714	Y. Fang, X. S. Zhang, J. L. Zhang, R. Z. Zhong and D. W. Zhou. Global DNA methylation and related mRNA profiles in sheep oocytes and early embryos derived from pre-pubertal and adult donors[J]. Animal Reproduction Science,2016,164:144-151.
APA	Y. Fang, X. S. Zhang, J. L. Zhang, R. Z. Zhong and D. W. Zhou.(2016).Global DNA methylation and related mRNA profiles in sheep oocytes and early embryos derived from pre-pubertal and adult donors.Animal Reproduction Science,164,144-151.
MLA	Y. Fang, X. S. Zhang, J. L. Zhang, R. Z. Zhong and D. W. Zhou."Global DNA methylation and related mRNA profiles in sheep oocytes and early embryos derived from pre-pubertal and adult donors".Animal Reproduction Science 164(2016):144-151.