De novo sequencing transcriptome of endemic Gentiana straminea (Gentianaceae) to identify genes involved in the biosynthesis of active ingredients

	De novo sequencing transcriptome of endemic Gentiana straminea (Gentianaceae) to identify genes involved in the biosynthesis of active ingredients
	Zhou, Dangwei 1,4; Gao, Shan 2; Wang, Huan 3; Lei, Tianxiang 1,4; Shen, Jianwei 3; Gao, Jie 1; Chen, Shilong1 ; Yin, Jia 1,4; Liu, Jianquan 1
刊名	gene
	2016
卷号	575 期号:1 页码:160-170
关键词	G. straminea Illumina sequencing Transcriptome Flavonoids Iridoid qRT-PCR SSR Qinghai-Tibet Plateau
英文摘要	gentiana straminea is a popular tibetan medicine that has been used for thousands of years in china to treat various diseases and conditions. although it has multiple pharmaceutical purposes and important economic plant resource in china, transcriptome and molecular base still known limited. in flowering season, samples were collected from different tissues, using the ngs illumina. solexa platform, about 58.85 million sequencing reads were generated and assembled de novo, yielding 78,764 high quality unigenes with an average length of 1090 bp. gene ontology (go), kegg pathway mapping showed that 49,033 of these were identified as putative homologs of annotated sequences in the protein databases. among them, candidate genes associated with iridoid, flavonoid and anthocyanin were identified. further the key enzymes involved to iridoid and flavonoid synthesis pathway were analyzed by quantitative real-time polymerase chain reaction (grt-pcr) on different tissues, the flower and root had the higher expression than leaves. in addition, 7591 ssr markers were identified from the unigenes of the g. straminea transcriptome. the foundation of g. straminea provided the important resource for facilitating to study molecular and functional genomics of it and related this species on the qinghai-tibet plateau. (c) 2015 elsevier b.v. all rights reserved.
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	genetics & heredity
研究领域[WOS]	genetics & heredity
关键词[WOS]	qinghai-tibetan plateau ; oleanolic acid ; rna-seq ; functional genomics ; ursolic acid ; markers ; constituents ; arabidopsis ; antioxidant ; macrophylla
收录类别	SCI
语种	英语
WOS记录号	WOS:000366226900020
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/6519]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota AEPB, Xining 810008, Qinghai, Peoples R China 2.Nankai Univ, Coll Life Sci, Tianjin 300071, Peoples R China 3.Chinese Acad Sci, Key Lab Tibetan Med, Xining 810008, Qinghai, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Dangwei,Gao, Shan,Wang, Huan,et al. De novo sequencing transcriptome of endemic Gentiana straminea (Gentianaceae) to identify genes involved in the biosynthesis of active ingredients[J]. gene,2016,575(1):160-170.
APA	Zhou, Dangwei.,Gao, Shan.,Wang, Huan.,Lei, Tianxiang.,Shen, Jianwei.,...&Liu, Jianquan.(2016).De novo sequencing transcriptome of endemic Gentiana straminea (Gentianaceae) to identify genes involved in the biosynthesis of active ingredients.gene,575(1),160-170.
MLA	Zhou, Dangwei,et al."De novo sequencing transcriptome of endemic Gentiana straminea (Gentianaceae) to identify genes involved in the biosynthesis of active ingredients".gene 575.1(2016):160-170.