Separation and purification of four tannins from Potentilla parvifolia Fisch (Rosaceae) using high-speed counter-current chromatography

	Separation and purification of four tannins from Potentilla parvifolia Fisch (Rosaceae) using high-speed counter-current chromatography
	Yuan, Zhenzhen 1,2,3; Lv, Huanhuan 1,3; Wang, Xiaoyan 1; Li, Gang 1; Suo, Yourui 1; Zhang, Zhang 4; Wang, Honglun 1,2
刊名	separation science and technology
	2016
卷号	51 期号:12 页码:2020-2027
关键词	HSCCC Potentilla parvifolia tannins proanthocyanidins preparative isolation
英文摘要	four tannins were isolated and identified from potentilla parvifolia using high-speed counter-current chromatography (hsccc) in this study. a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (1:11:1.2:11, v/v/v/v) was chosen and yielded 18.8 mg of procyanidin b3, 9.8 mg of procyanidin b6, 9.1 mg of procyanidin b7, and 6.9 mg of 2,3,4,6-tetra-o-galloyl-d-glucose, all at over 95% purity, from 120 mg of the crude sample powder. the identifications were performed with h-1 nmr and c-13 nmr. this is the first report showing the presence of procyanidin b7 and 2,3,4,6-tetra-o-galloyl-d-glucose in the genus potentilla, and procyanidin b3 and procyanidin b6 were isolated from p. parvifolia for the first time.
WOS标题词	science & technology ; physical sciences ; technology
类目[WOS]	chemistry, multidisciplinary ; engineering, chemical
研究领域[WOS]	chemistry ; engineering
关键词[WOS]	in-vitro ; hydrolyzable tannins ; terminalia-chebula ; glucosides ; extracts ; fruits
收录类别	SCI
语种	英语
WOS记录号	WOS:000382372100009
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/6447]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Peoples R China 2.Chinese Acad Sci, Huzhou Plateau Biol Resource Ctr Innovat, Northwest Inst Plateau Biol, Huzhou, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.JALA Co Ltd, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Yuan, Zhenzhen,Lv, Huanhuan,Wang, Xiaoyan,et al. Separation and purification of four tannins from Potentilla parvifolia Fisch (Rosaceae) using high-speed counter-current chromatography[J]. separation science and technology,2016,51(12):2020-2027.
APA	Yuan, Zhenzhen.,Lv, Huanhuan.,Wang, Xiaoyan.,Li, Gang.,Suo, Yourui.,...&Wang, Honglun.(2016).Separation and purification of four tannins from Potentilla parvifolia Fisch (Rosaceae) using high-speed counter-current chromatography.separation science and technology,51(12),2020-2027.
MLA	Yuan, Zhenzhen,et al."Separation and purification of four tannins from Potentilla parvifolia Fisch (Rosaceae) using high-speed counter-current chromatography".separation science and technology 51.12(2016):2020-2027.