Characterization of a Metagenome-Derived beta-Glucosidase and Its Application in Conversion of Polydatin to Resveratrol

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 中科院海洋生物资源可持续利用重点实验室

	Characterization of a Metagenome-Derived beta-Glucosidase and Its Application in Conversion of Polydatin to Resveratrol
	Mai, Zhimao; Su, Hongfei; Zhang, Si
刊名	CATALYSTS
	2016
卷号	6 期号:3 页码:35
关键词	beta-glucosidase mangrove soil metagenomic library polydatin resveratrol
通讯作者	Zhang, S (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.
中文摘要	For the beneficial pharmacological properties of resveratrol, there is increasingly interest in enzymatic conversion of polydatin to resveratrol. The metagenomic technique provides an effective strategy for mining novel polydatin-hydrolysis enzymes from uncultured microorganisms. In this study, a metagenomic library of mangrove soil was constructed and a novel -glucosidase gene MlBgl was isolated. The deduced amino acid sequences of MlBgl showed the highest identity of 64% with predicted -glucosidase in the GenBank database. The gene was cloned and overexpressed in Escherichia coli BL21(DE3). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) assay demonstrated the purified recombinant -glucosidase r-MlBgl with a molecular weight approximately of 71 kDa. The optimal pH and temperature of purified recombinant r-MlBgl were 7.0 and 40 degrees C, respectively. r-MlBgl could hydrolyze polydatin effectively. The k(cat) and k(cat)/K-m values for polydatin were 989 s(-1) and 1476 mM(-1)s(-1), respectively. These properties suggest that -r-MlBgl has potential application in the enzymatic conversion of polydatin to resveratrol for further study.
学科主题	Chemistry
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/15483]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	Mai, Zhimao,Su, Hongfei,Zhang, Si. Characterization of a Metagenome-Derived beta-Glucosidase and Its Application in Conversion of Polydatin to Resveratrol[J]. CATALYSTS,2016,6(3):35.
APA	Mai, Zhimao,Su, Hongfei,&Zhang, Si.(2016).Characterization of a Metagenome-Derived beta-Glucosidase and Its Application in Conversion of Polydatin to Resveratrol.CATALYSTS,6(3),35.
MLA	Mai, Zhimao,et al."Characterization of a Metagenome-Derived beta-Glucosidase and Its Application in Conversion of Polydatin to Resveratrol".CATALYSTS 6.3(2016):35.