Molecular cloning, inducible expression and antibacterial analysis of a novel i-type lysozyme (lyz-i2) in Pacific white shrimp, Litopenaeus vannamei

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	Molecular cloning, inducible expression and antibacterial analysis of a novel i-type lysozyme (lyz-i2) in Pacific white shrimp, Litopenaeus vannamei
	Chen, Ting; Ren, Chunhua; Wang, Yanhong; Luo, Peng; Jiang, Xiao; Huang, Wen; Chen, Chang; Hu, Chaoqun
刊名	FISH & SHELLFISH IMMUNOLOGY
	2016
卷号	54 页码:197-203
关键词	I-type lysozyme Litopenaeus vannamei Antimicrobial activity Innate immunity Vibrio
通讯作者	Ren, CH ; Hu, CQ (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol LAMB, Key Lab Trop Marine Bioresources & Ecol LMB, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China.
中文摘要	The full-length cDNA coding for a novel invertebrate (i-type) lysozyme was identified in Pacific white shrimp (Litopenaeus vannamei). The newly obtained L. vannamei lysozyme is similar to the Penaeus monodon i-type lysozyme 2, but it is distant from the known L vannamei c-type lysozyme and i-type lysozyme 1 in protein sequence; therefore, it was defined as L vannamei i-type lysozyme 2 (lyz-i2). Expression of L. vannamei lyz-i2 transcripts were ubiquitously detected in all tissues we selected, with the highest abundance observed in the hemolymph. Challenge with Vibrio harveyi might elicit L vannamei lyz-i2 mRNA expression in the hepatopancreas, intestine, muscle, gill and hemolymph. In the themolymph, specifically, the stimulatory effects of Vibrio and lipopolysaccharide (LPS) on lyz-i2 transcript levels were durable and transient, respectively; while Polyinosinic:polycytidylic acid [Poly (I:C)] treatment did not affect lyz-i2 expression. L vannamei lyz-i2 recombinant protein was generated in an Escherichia coli system. By lysoplate and turbidimetric assays, the L vannamei lyz-i2 recombinant protein showed a broad spectrum of antimicrobial properties with high activities against Micrococcaceae lysodeikticus and various Vibrio species and relatively low activity against E. coll. In conclusion, L. vannamei lyz-i2 might be a potent antibacterial protein with a role in innate immunity in Penaeid shrimp. (C) 2016 Elsevier Ltd. All rights reserved.
学科主题	Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/15388]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	Chen, Ting,Ren, Chunhua,Wang, Yanhong,et al. Molecular cloning, inducible expression and antibacterial analysis of a novel i-type lysozyme (lyz-i2) in Pacific white shrimp, Litopenaeus vannamei[J]. FISH & SHELLFISH IMMUNOLOGY,2016,54:197-203.
APA	Chen, Ting.,Ren, Chunhua.,Wang, Yanhong.,Luo, Peng.,Jiang, Xiao.,...&Hu, Chaoqun.(2016).Molecular cloning, inducible expression and antibacterial analysis of a novel i-type lysozyme (lyz-i2) in Pacific white shrimp, Litopenaeus vannamei.FISH & SHELLFISH IMMUNOLOGY,54,197-203.
MLA	Chen, Ting,et al."Molecular cloning, inducible expression and antibacterial analysis of a novel i-type lysozyme (lyz-i2) in Pacific white shrimp, Litopenaeus vannamei".FISH & SHELLFISH IMMUNOLOGY 54(2016):197-203.