The leptin system and its expression at different nutritional and pregnant stages in lined seahorse (Hippocampus erectus)

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	The leptin system and its expression at different nutritional and pregnant stages in lined seahorse (Hippocampus erectus)
	Zhang, Huixian; Qin, Geng; Zhang, Yanhong; Li, Shuisheng; Lin, Qiang
刊名	BIOLOGY OPEN
	2016
卷号	5 期号:10 页码:1508-1515
关键词	Leptin Leptin receptor Nutrient Pregnancy Seahorse
通讯作者	Lin, Q (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.
英文摘要	Leptin is an essential hormone for the regulation of energy metabolism and food intake in vertebrate animals. To better understand the physiological roles of leptin in nutrient regulation in paternal ovoviviparous fish (family Syngnathidae), the present study cloned the full-length of leptin-a and leptin receptor (lepr) genes in lined seahorse (Hippocampus erectus). Results showed that there was a 576-bp intron between two exons in leptin-a gene but no leptin-b gene in seahorse. Although the primary amino acid sequence conservation of seahorse leptin-a was very low, the 3-D structure modeling of seahorse leptin-a revealed strong conservation of tertiary structure with other vertebrates. Seahorse leptin-a mRNA was highly expressed in brain, whereas lepr mRNA was mainly expressed in ovary and gill. Interestingly, both leptin-a and lepr mRNA were expressed in the brood pouch of male seahorse, suggesting the leptin system plays a role during the male pregnancy. Physiological experiments showed that the expression of hepatic leptin-a and lepr mRNA in unfed seahorses was significantly higher than that in those fed 100%, as well as 60%, of their food during the fasting stage, showing that seahorse might initiate the leptin system to regulate its energy metabolism while starving. Moreover, the expression of leptin-a in the brood pouch of pregnant seahorse was significantly upregulated compared with non-pregnant seahorse, whereas the expression of lepr was downregulated, suggesting that the leptin system might be involved in the male pregnancy. In conclusion, the leptin system plays a role in the energy metabolism and food intake, and might provide new insights into molecular regulation of male pregnancy in seahorse.
学科主题	Life Sciences & Biomedicine - Other Topics
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/15273]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	Zhang, Huixian,Qin, Geng,Zhang, Yanhong,et al. The leptin system and its expression at different nutritional and pregnant stages in lined seahorse (Hippocampus erectus)[J]. BIOLOGY OPEN,2016,5(10):1508-1515.
APA	Zhang, Huixian,Qin, Geng,Zhang, Yanhong,Li, Shuisheng,&Lin, Qiang.(2016).The leptin system and its expression at different nutritional and pregnant stages in lined seahorse (Hippocampus erectus).BIOLOGY OPEN,5(10),1508-1515.
MLA	Zhang, Huixian,et al."The leptin system and its expression at different nutritional and pregnant stages in lined seahorse (Hippocampus erectus)".BIOLOGY OPEN 5.10(2016):1508-1515.