Isolation and function analysis of apolipoprotein A-I gene response to virus infection in grouper

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	Isolation and function analysis of apolipoprotein A-I gene response to virus infection in grouper
	Wei, Jingguang 1; Gao, Pin 1; Zhang, Ping 1; Guo, Minglan 1; Xu, Meng 1; Wei, Shina 1; Yan, Yang 1; Qin, Qiwei 1
刊名	FISH & SHELLFISH IMMUNOLOGY
	2015
卷号	43 期号:2 页码:396-404
关键词	Epinephelus coioides Apolipoprotein A-I Innate immunity Intracellular localization
通讯作者	qinqw@scsio.ac.cn
英文摘要	Apolipoproteins, synthesized mainly in liver and intestine and bounded to lipids, play important roles in lipid transport and uptake through the circulation system. In this study, an apolipoprotein A-I gene homologue was cloned from orange-spotted grouper Epinephelus coioides (designed as Ec-ApoA-I) by rapid amplification of cDNA ends (RACE) PCR. The full-length cDNA of Ec-ApoA-I was comprised of 1278 bp with a 792 bp open reading frame (ORF) that encodes a putative protein of 264 amino acids. Quantitative real-time PCR (qPCR) analysis revealed that Ec-ApoA-I was abundant in liver and intestine, and the expression in liver was significantly (P < 0.01) up-regulated after the stimulation of LPS, Poly(I:C), Vibrio alginolyticus, and Singapore grouper iridovirus (SGIV). Recombinant Ec-ApoA-I (rEc-ApoA-I) was produced in Escherichia coli BL21 (DE3) expression system exhibited bacteriolyticactivity against Microcococcus lysodeikticus and Aeromonas hydrophila. Intracellular localization revealed that Ec-ApoA-I distributed in both cytoplasm and nucleus, and predominantly in the cytoplasm. Overexpression of Ec-ApoA-I in grouper Brain (GB) cells could inhibit the replication of SGIV. These results together indicated that Ec-ApoA-I perhaps is involved in the responses to bacterial and viral challenge. (C) 2015 Elsevier Ltd. All rights reserved.
学科主题	Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
原文出处	1050-4648
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/14846]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
作者单位	1.[Wei, Jingguang 2.Guo, Minglan 3.Wei, Shina 4.Yan, Yang 5.Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China 6.[Wei, Jingguang 7.Guo, Minglan 8.Wei, Shina 9.Yan, Yang 10.Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol, Guangzhou 510301, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Wei, Jingguang,Gao, Pin,Zhang, Ping,et al. Isolation and function analysis of apolipoprotein A-I gene response to virus infection in grouper[J]. FISH & SHELLFISH IMMUNOLOGY,2015,43(2):396-404.
APA	Wei, Jingguang.,Gao, Pin.,Zhang, Ping.,Guo, Minglan.,Xu, Meng.,...&Qin, Qiwei.(2015).Isolation and function analysis of apolipoprotein A-I gene response to virus infection in grouper.FISH & SHELLFISH IMMUNOLOGY,43(2),396-404.
MLA	Wei, Jingguang,et al."Isolation and function analysis of apolipoprotein A-I gene response to virus infection in grouper".FISH & SHELLFISH IMMUNOLOGY 43.2(2015):396-404.