Expression profiles and interaction suggest TBK1 can be regulated by Nrdp1 in response to immune stimulation in large yellow croaker Larimichthys crocea

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	Expression profiles and interaction suggest TBK1 can be regulated by Nrdp1 in response to immune stimulation in large yellow croaker Larimichthys crocea
	Zhang, Dong Ling 1; Yu, Da Hui 1; Chen, Jian 1; Fan, Sigang 1; Wang, Zhi Yong 1
刊名	FISH & SHELLFISH IMMUNOLOGY
	2015
卷号	46 期号:2 页码:745-752
关键词	Larimichthys crocea TBK1 Nrdp1 Protein interaction Immune stimulation
通讯作者	zywang@jmu.edu.cn
英文摘要	TBK1 has been extensively studied in mammals because of its important roles as a molecular bridge, linking the TLRs (TLR3 and TLR4) and RLRs signals to activate transcriptional factors IRF3 and IRF7 for IFN-I production. However, the information on molecular and functional characteristics of TBK1 in teleosts is limited. In this study, the molecular characterization and immune response of TBK1 in Larimichthys crocea (named as LcTBK1) as well as its interaction with Nrdp1 were investigated. Sequence analysis demonstrated that LcTBK1 included four functional motifs, the N-terminal protein kinase domain and ATP-binding site, middle ULD and C-terminal coiled-coil domain. The tissue expression profiles indicated that LcTBK1 gene was constitutively expressed in the twelve tissues examined, with high expression in brain. Temporal expression analysis showed that LcTBK1 mRNA was obviously increased in the liver after injection of LPS, Poly I:C and inactive Vibrio parahaemolyticus, however, declined at some time points in spleen and head-kidney. Furthermore, we found that LcTBK1 can interact with LcNrdp1, an E3 ubiquitin ligase that involved in immune response to Cryptoccuyon irritans infection in L. crocea. The qPCR showed that LcNrdp1 was also significantly up-regulated in liver, down-regualted at some time points in spleen and head-kidney after LPS, Poly I:C and inactive V. parahaemolyticus injection, although the expression patterns of the two genes after the three treatments were different in change magnitude and up-regulation timespan. These results suggested that LcTBK1 was involved in L. crocea defense against the pathogen infection and can be regulated by Nrdp1 in PPRs signaling pathway of fishes. (C) 2015 Elsevier Ltd. Alt rights reserved.
学科主题	Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
原文出处	1050-4648
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/14763]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
作者单位	1.[Zhang, Dong Ling 2.Chen, Jian 3.Wang, Zhi Yong] Jimei Univ, Coll Fisheries, Key Lab Hlth Mariculture East China Sea, Minist Agr, Xiamen 361021, Fujian Province, Peoples R China 4.[Yu, Da Hui 5.Fan, Sigang] Chinese Acad Fishery Sci, South China Sea Resource Exploitat & Protect Coll, South China Sea Fisheries Res Inst, Guangzhou 510300, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Dong Ling,Yu, Da Hui,Chen, Jian,et al. Expression profiles and interaction suggest TBK1 can be regulated by Nrdp1 in response to immune stimulation in large yellow croaker Larimichthys crocea[J]. FISH & SHELLFISH IMMUNOLOGY,2015,46(2):745-752.
APA	Zhang, Dong Ling,Yu, Da Hui,Chen, Jian,Fan, Sigang,&Wang, Zhi Yong.(2015).Expression profiles and interaction suggest TBK1 can be regulated by Nrdp1 in response to immune stimulation in large yellow croaker Larimichthys crocea.FISH & SHELLFISH IMMUNOLOGY,46(2),745-752.
MLA	Zhang, Dong Ling,et al."Expression profiles and interaction suggest TBK1 can be regulated by Nrdp1 in response to immune stimulation in large yellow croaker Larimichthys crocea".FISH & SHELLFISH IMMUNOLOGY 46.2(2015):745-752.