3-Methyl-1-butanol Biosynthesis in an Engineered Corynebacterium glutamicum

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	3-Methyl-1-butanol Biosynthesis in an Engineered Corynebacterium glutamicum
	Xiao, Shiyuan 1,2; Xu, Jingliang1 ; Chen, Xiaoyan 1; Li, Xiekun 1,2; Zhang, Yu1 ; Yuan, Zhenhong1
刊名	molecular biotechnology
	2016-05-01
卷号	58 期号:5 页码:311-318
关键词	3-Methyl-1-butanol Corynebacterium glutamicum alpha-Ketoisovalerate decarboxylase Alcohol dehydrogenase Gene knockout
英文摘要	biofuel offers a promising solution to the adverse environmental problems and depletion in reserves of fossil fuels. higher alcohols including 3-methyl-1-butanol were paid much more attention as fuel substitute in recent years, due to its similar properties to gasoline. in the present work, 3-methyl-1-butanol production in engineered corynebacterium glutamicum was studied. alpha-ketoisovalerate decarboxylase gene (kivd) from lactococcus lactis combined with alcohol dehydrogenase gene (adh2, adha, and adh3) from three organisms were overexpressed in c. glutamicum. enzymatic assay and alcohol production results showed that adh3 from zymomonas mobilis was the optimum candidate for 3-methyl-1-butanol production in c. glutamicum. the recombinant with kivd and adh3 could produce 0.182 g/l of 3-methyl-1-butanol and 0.144 g/l of isobutanol after 12 h of incubation. further inactivation of the e1 subunit of pyruvate dehydrogenase complex gene (acee) and lactic dehydrogenase gene (ldh) in the above c. glutamicum strain would improve the 3-methyl-1-butanol titer to 0.497 g/l after 12 h of incubation.
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biochemistry & molecular biology ; biotechnology & applied microbiology
研究领域[WOS]	biochemistry & molecular biology ; biotechnology & applied microbiology
关键词[WOS]	pyruvate-dehydrogenase complex ; escherichia-coli ; amino-acids ; decarboxylases ; fermentation ; mutagenesis ; alcohols ; enzyme ; gene
收录类别	SCI
语种	英语
WOS记录号	WOS:000374286500001
内容类型	期刊论文
源URL	[http://ir.giec.ac.cn/handle/344007/11237]
专题	中国科学院广州能源研究所
作者单位	1.Chinese Acad Sci, Guangzhou Inst Energy Convers, Key Lab Renewable Energy, Guangzhou, Guangdong, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Xiao, Shiyuan,Xu, Jingliang,Chen, Xiaoyan,et al. 3-Methyl-1-butanol Biosynthesis in an Engineered Corynebacterium glutamicum[J]. molecular biotechnology,2016,58(5):311-318.
APA	Xiao, Shiyuan,Xu, Jingliang,Chen, Xiaoyan,Li, Xiekun,Zhang, Yu,&Yuan, Zhenhong.(2016).3-Methyl-1-butanol Biosynthesis in an Engineered Corynebacterium glutamicum.molecular biotechnology,58(5),311-318.
MLA	Xiao, Shiyuan,et al."3-Methyl-1-butanol Biosynthesis in an Engineered Corynebacterium glutamicum".molecular biotechnology 58.5(2016):311-318.