Characterization of Two Human Lung Adenocarcinoma Cell Lines by Reciprocal Chromosome Painting

CORC > 昆明动物研究所 > 昆明动物研究所 > 遗传资源与进化国家重点实验室 > 保护遗传学(细胞库)

	Characterization of Two Human Lung Adenocarcinoma Cell Lines by Reciprocal Chromosome Painting
	Peng KJ 1,4; Wang JH 1; Su WT 1; Wang XC 2; Yang FT 3; Nie WH[*]1
刊名	Zoological Research
	2010
卷号	31 期号:2 页码:113-121
关键词	Lung adenocarcinoma cell lines Chromosome sorting Chromosome painting Cytogenetic characterization
其他题名	利用相互染色体涂色技术分析两株人肺腺癌细胞系(A549和GLC-82)的核型特征
通讯作者	whnie@mail.kiz.ac.cn
合作状况	其它
中文摘要	肺癌是全世界癌症死亡中的一个主要的原因。除吸烟外,一些肺癌患者的发病与氡气污染相关。该研究采用包括染色体分选、正向和反向染色体涂色技术,分析了两株肺腺癌细胞系A549和GLC-82的核型特征。A549和GLC-82细胞系都属于非小细胞肺癌细胞系,但诱因不同,后者来源于一个长期生活在氡气污染环境肺癌病人的癌组织。染色体涂色结果表明,这两株肺癌细胞系发生了复杂的染色体重排。在A549和GLC-82细胞系中,除正常染色体拷贝数变化外,还分别存在13条和24条畸变染色体。约一半的畸变染色体是通过非相互易位形成的,其余的畸变染色体则是通过一些正常染色体的片段缺失或重复而产生的。尽管这两株肺癌细胞系都没有共同的畸变染色体,但它们似共享两个染色体易位断裂点:HSA8q24和12q14。
英文摘要	Lung cancer is a leading cause of cancer death worldwide. Some lung cancer patients correlate with a gas of radon besides smoking. To search for common chromosomal aberrations in lung cancer cell lines established from patients induced by different factors, a combined approach of chromosome sorting, forward and reverse chromosome painting was used to characterize karyotypes of two lung adenocarcinoma cell lines: A549 and GLC-82 with the latter line derived from a patient who has suffered long-term exposure to environmental radon gas pollution. The chromosome painting results revealed that complex chromosomal rearrangements occurred in these two lung adenocarcinoma cell lines. Thirteen and twenty-four abnormal chromosomes were identified in A549 and GLC-82 cell lines, respectively. Almost half of abnormal chromosomes in these two cell lines were formed by non-reciprocal translocations, the others were derived from deletions and duplication/or amplification in some chromosomal regions. Furthermore, two apparently common breakpoints, HSA8q24 and 12q14 were found in these two lung cancer cell lines.
收录类别	其他
资助信息	This study is supported partly by grants from the Ministry of Science and Technology of China (2005DKA21502); the Joint Foundat
语种	英语
公开日期	2010-08-19
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/1697]
专题	昆明动物研究所_保护遗传学(细胞库) 昆明动物研究所_遗传资源与进化国家重点实验室
作者单位	1.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, the Chinese Academy of Sciences ， Kunming 650223, China 2.Tumor Hospital and Tumor Institute of Yunnan Province ， the Third Affiliated Hospital of Kunming Medical University, , Kunming 650118, China 3.Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK 4.The Graduate School of the Chinese Academy of Sciences, Beijing 100049, China
推荐引用方式 GB/T 7714	Peng KJ,Wang JH,Su WT,et al. Characterization of Two Human Lung Adenocarcinoma Cell Lines by Reciprocal Chromosome Painting[J]. Zoological Research,2010,31(2):113-121.
APA	Peng KJ,Wang JH,Su WT,Wang XC,Yang FT,&Nie WH[*].(2010).Characterization of Two Human Lung Adenocarcinoma Cell Lines by Reciprocal Chromosome Painting.Zoological Research,31(2),113-121.
MLA	Peng KJ,et al."Characterization of Two Human Lung Adenocarcinoma Cell Lines by Reciprocal Chromosome Painting".Zoological Research 31.2(2010):113-121.