Protein Pretreatment of Microelectrodes Enables in Vivo Electrochemical Measurements with Easy Precalibration and Interference-Free from Proteins

	Protein Pretreatment of Microelectrodes Enables in Vivo Electrochemical Measurements with Easy Precalibration and Interference-Free from Proteins
	Liu, Xiaomeng 1; Zhang, Meining 1; Xiao, Tongfang 2; Hao, Jie 2; Li, Ruixin 1; Mao, Lanqun 2
刊名	ANALYTICAL CHEMISTRY
	2016-07-19
卷号	88 期号:14 页码:7238-7244
英文摘要	In vivo electrochemistry is one powerful strategy for probing brain chemistry. However, the decreases in sensitivity mainly caused by the adsorption of proteins onto electrode surface in short-term in vivo measurements unfortunately render great challenges in both electrode calibration and selectivity against the alternation of proteins. In this study, we observe that the pretreatment of carbon fiber microelectrodes (CFEs) with bovine serum albumin (BSA) would offer a simple but effective strategy to the challenges mentioned above. We verify our strategy for dopamine (DA) with conventionally used CFEs and for ascorbate with our previously developed carbon nanotube-modified CFEs. We find that, in artificial cerebral spinal fluid (aCSF) solution containing BSA, the current responses of the microelectrodes equilibrate shortly and the results for precalibration carried out in this solution are found to be almost the same as those for the postcalibration in pure aCSF. This observation offers a new solution to electrode calibration for in vivo measurements with a technical simplicity. Furthermore, we find that the use of BSA pretreated CFEs to replace bare CFEs would minimize the interference from the alternation of proteins in the brain. This study offers a new general and effective approach to in vivo electrochemistry with a high reliability and a simplified procedure.
收录类别	SCI
语种	英语
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/35202]
专题	化学研究所_活体分析化学实验室
作者单位	1.Renmin Univ China, Dept Chem, Beijing 100872, Peoples R China 2.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Liu, Xiaomeng,Zhang, Meining,Xiao, Tongfang,et al. Protein Pretreatment of Microelectrodes Enables in Vivo Electrochemical Measurements with Easy Precalibration and Interference-Free from Proteins[J]. ANALYTICAL CHEMISTRY,2016,88(14):7238-7244.
APA	Liu, Xiaomeng,Zhang, Meining,Xiao, Tongfang,Hao, Jie,Li, Ruixin,&Mao, Lanqun.(2016).Protein Pretreatment of Microelectrodes Enables in Vivo Electrochemical Measurements with Easy Precalibration and Interference-Free from Proteins.ANALYTICAL CHEMISTRY,88(14),7238-7244.
MLA	Liu, Xiaomeng,et al."Protein Pretreatment of Microelectrodes Enables in Vivo Electrochemical Measurements with Easy Precalibration and Interference-Free from Proteins".ANALYTICAL CHEMISTRY 88.14(2016):7238-7244.