Single-molecule force spectroscopy study of the effect of cigarette carcinogens on thrombomodulin-thrombin interaction

CORC > 化学研究所 > 中国科学院化学研究所 > 分子纳米结构与纳米技术实验室

	Single-molecule force spectroscopy study of the effect of cigarette carcinogens on thrombomodulin-thrombin interaction
	Liu, Jianli 1; Zhang, Xuejie 1,2; Wang, Xiaofeng 2; Xu, Li 1; Li, Jingyuan 2; Fang, Xiaohong 1
刊名	SCIENCE BULLETIN
	2016-08-01
卷号	61 期号:15 页码:1187-1194
关键词	Cigarette carcinogens Thrombin Thrombomodulin AFM Single-molecule force spectroscopy Molecular simulation
英文摘要	Exposure to cigarette smoke is a major risk factor for cancer and cardiovascular disease. Thrombosis is regarded as the main reason for smoking-related cardiovascular disease. However, the detail mechanism of how smoking promotes thrombosis is not fully understood. In this work, we investigated the impacts of one major cigarette carcinogens 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) as well as its metabolite 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) on a key process in thrombosis regulation: thrombin-thrombomodulin (TM) binding. Atomic force microscopy based single-molecule force spectroscopy was applied to measure both in vitro and in vivo binding force of thrombin to TM in the absence and presence of NNK and NNAL respectively. The results revealed that NNK and NNAL can reduce the binding probability of TM and thrombin. The inhibition effect and underlying mechanism was further studied by molecular simulation. As indicated by our results, the cigarette carcinogens could cause a higher risk of thrombosis through the disruption of TM-thrombin interaction.
收录类别	SCI
语种	英语
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/35220]
专题	化学研究所_分子纳米结构与纳米技术实验室
作者单位	1.Chinese Acad Sci, Inst Chem, Key Lab Mol Nanostruct & Nanotechnol, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Inst High Energy Phys, Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Liu, Jianli,Zhang, Xuejie,Wang, Xiaofeng,et al. Single-molecule force spectroscopy study of the effect of cigarette carcinogens on thrombomodulin-thrombin interaction[J]. SCIENCE BULLETIN,2016,61(15):1187-1194.
APA	Liu, Jianli,Zhang, Xuejie,Wang, Xiaofeng,Xu, Li,Li, Jingyuan,&Fang, Xiaohong.(2016).Single-molecule force spectroscopy study of the effect of cigarette carcinogens on thrombomodulin-thrombin interaction.SCIENCE BULLETIN,61(15),1187-1194.
MLA	Liu, Jianli,et al."Single-molecule force spectroscopy study of the effect of cigarette carcinogens on thrombomodulin-thrombin interaction".SCIENCE BULLETIN 61.15(2016):1187-1194.