Improved performance of immobilized laccase on amine-functioned magnetic Fe3O4 nanoparticles modified with polyethylenimine

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	Improved performance of immobilized laccase on amine-functioned magnetic Fe3O4 nanoparticles modified with polyethylenimine
	Xia, Ting-Ting ; Liu, Chun-Zhao 1; Hu, Jian-Hua ; Guo, Chen 1
刊名	CHEMICAL ENGINEERING JOURNAL
	2016-07-01
卷号	295 期号:JULY 页码:201-206
关键词	Amine-functioned Fe3O4 nanoparticle Polyethylenimine Spacer-arm Cu2+ chelated Laccase Immobilization
ISSN号	1385-8947
英文摘要	Polyethylenimine (PEI) as a spacer-arm polymer was modified on amine-functioned Fe3O4 nanoparticles (Fe3O4-NH2 NPs). PEI modified Fe3O4 nanoparticles (Fe304-NH2-PEI NPs) were chelated with Cu2+ to immobilize laccase through metal affinity adsorption. Meanwhile, PEI un-modified Fe3O4-NH2 NPs acted as a control group. The adsorption capacity of Fe304-NH2-PEI-Cu2+ NPs was larger than Fe3O4-NH2-Cu2+ NPs almost in the whole range of laccase concentration. And the activity recovery of Fe3O4-NH2-PEILaccase was also higher than Fe304-NH2-Laccase when the laccase concentration was lower than 26 pg/mL. The maximum activity recovery of Fe3O4-NH2-PEI-Laccase (107.41%) was much higher than Fe3O4-NH2-Laccase (42.75%). The corresponding specific activity of Fe3O4-NH2-PEI-Laccase and Fe3O4-NH2-Laccase was separately 101.33 and 74.45 times as large as free laccase product. The enzymatic properties of the two immobilized laccases were both improved and the immobilization realized reuse of laccase. The results showed that Fe3O4-NH2-PEI-Cu2+ NPs were more promising than Fe3O4NH2-Cu2+ NPs for purification and immobilization of laccase simultaneously and are potential for large-scale laccase immobilization. (C) 2016 Elsevier B.V. All rights reserved.
WOS标题词	Science & Technology ; Technology
类目[WOS]	Engineering, Environmental ; Engineering, Chemical
研究领域[WOS]	Engineering
关键词[WOS]	CHELATE AFFINITY INTERACTION ; TRAMETES-VERSICOLOR ; PHENOLIC-COMPOUNDS ; REVERSIBLE IMMOBILIZATION ; COMPOSITE ; DEGRADATION ; PARTICLES ; REMOVAL ; DYE ; CHITOSAN
收录类别	SCI
语种	英语
WOS记录号	WOS:000375507300022
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/21072]
专题	过程工程研究所_生化工程国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Xia, Ting-Ting,Liu, Chun-Zhao,Hu, Jian-Hua,et al. Improved performance of immobilized laccase on amine-functioned magnetic Fe3O4 nanoparticles modified with polyethylenimine[J]. CHEMICAL ENGINEERING JOURNAL,2016,295(JULY):201-206.
APA	Xia, Ting-Ting,Liu, Chun-Zhao,Hu, Jian-Hua,&Guo, Chen.(2016).Improved performance of immobilized laccase on amine-functioned magnetic Fe3O4 nanoparticles modified with polyethylenimine.CHEMICAL ENGINEERING JOURNAL,295(JULY),201-206.
MLA	Xia, Ting-Ting,et al."Improved performance of immobilized laccase on amine-functioned magnetic Fe3O4 nanoparticles modified with polyethylenimine".CHEMICAL ENGINEERING JOURNAL 295.JULY(2016):201-206.