Thermo- and pH-responsive polymer brushes-grafted gigaporous polystyrene microspheres as a high-speed protein chromatography matrix

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	Thermo- and pH-responsive polymer brushes-grafted gigaporous polystyrene microspheres as a high-speed protein chromatography matrix
	Qu, Jian-Bo 1; Xu, Yu-Liang 1; Liu, Jun-Yi 1; Zeng, Jing-Bin 1; Chen, Yan-Li 1; Zhou, Wei-Qing 2; Liu, Jian-Guo 1
刊名	JOURNAL OF CHROMATOGRAPHY A
	2016-04-08
卷号	1441 期号:APR 页码:60-67
关键词	Gigaporous polystyrene microspheres Thermo- and pH-responsive polymer brushes Protein separation High-speed protein chromatography Atom transfer radical polymerization
ISSN号	0021-9673
英文摘要	Dual thermo- and pH-responsive chromatography has been proposed using poly(N-isopropylacrylamid-eco-butyl methacrylate-co-N,N-dimethylaminopropyl acrylamide) (P(NIPAM-co-BMA-co-DMAPAAM)) brushes grafted gigaporous polystyrene microspheres (GPM) as matrix. Atom transfer radical polymerization (ATRP) initiator was first coupled onto GPM through Friedel-Crafts acylation with 2-bromoisobutyryl bromide. The dual-responsive polymer brushes were then grafted onto GPM via surface-initiated ATRP. The surface composition, gigaporous structure, protein adsorption and dual-responsive chromatographic properties of the matrix (GPM-P(NIPAM-co-BMA-co-DMAPAAM) were characterized in detail. Results showed that GPM were successfully grafted with thermoresponsive cationic polymer brushes and that the gigaporous structure was well maintained. A column packed with GPM-P(NIPAM-co-BMA-co-DMAPAAM presented low backpressure, good permeability and appreciable thermo-responsibility. By changing pH of the mobile phase and temperature of the column in turn, the column can separate three model proteins at the mobile phase velocity up to 2528 cm h(-1). A separation mechanism of this matrix was also proposed. All results indicate that the dual thermo- and pH-responsive chromatography matrix has great potentials in 'green' high-speed protein chromatography. (C) 2016 Elsevier B.V. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]	Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]	Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]	STATIONARY-PHASE ; PERFUSION CHROMATOGRAPHY ; AQUEOUS CHROMATOGRAPHY ; LIQUID-CHROMATOGRAPHY ; SEPARATION ; PEPTIDES ; BIOMOLECULES ; ADSORPTION ; SUPPORTS ; LIGAND
收录类别	SCI
语种	英语
WOS记录号	WOS:000373552300008
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/20990]
专题	过程工程研究所_生化工程国家重点实验室
作者单位	1.China Univ Petr East China, Ctr Bioengn & Biotechnol, State Key Lab Heavy Oil Proc, Qingdao 266580, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Qu, Jian-Bo,Xu, Yu-Liang,Liu, Jun-Yi,et al. Thermo- and pH-responsive polymer brushes-grafted gigaporous polystyrene microspheres as a high-speed protein chromatography matrix[J]. JOURNAL OF CHROMATOGRAPHY A,2016,1441(APR):60-67.
APA	Qu, Jian-Bo.,Xu, Yu-Liang.,Liu, Jun-Yi.,Zeng, Jing-Bin.,Chen, Yan-Li.,...&Liu, Jian-Guo.(2016).Thermo- and pH-responsive polymer brushes-grafted gigaporous polystyrene microspheres as a high-speed protein chromatography matrix.JOURNAL OF CHROMATOGRAPHY A,1441(APR),60-67.
MLA	Qu, Jian-Bo,et al."Thermo- and pH-responsive polymer brushes-grafted gigaporous polystyrene microspheres as a high-speed protein chromatography matrix".JOURNAL OF CHROMATOGRAPHY A 1441.APR(2016):60-67.