Radiolabeling of DNA Bipyramid and Preliminary Biological Evaluation in Mice

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	Radiolabeling of DNA Bipyramid and Preliminary Biological Evaluation in Mice
	Li, JB; Jiang, DW; Bao, BL; He, YL; Liu, L; Wang, XM
刊名	BIOCONJUGATE CHEMISTRY
	2016
卷号	27 期号:4 页码:905—910
关键词	IN-VIVO PRECLINICAL EVALUATION NANOSTRUCTURES DELIVERY PLATFORM CANCER CELLS PET
ISSN号	1043-1802
通讯作者	Li, JB ; Wang, XM (reprint author), Inner Mongolia Med Univ, Dept Nucl Med, Affiliated Hosp, 1 Tongdao North St, Hohhot 010050, Peoples R China.
英文摘要	Self-assembled DNA nanostructures, as a new type of nanocarriers, have shown great potential in molecular imaging probes. DNA bipyramid nanostructures (DBNs), as a classic kind of DNA nanostructure, can be precisely constructed through the unparalleled base-pairing precision of oligonucleotide strands. DBNs were prepared by self-assembly of six oligonucleotides with equal molar amounts in a single annealing step, and purified by high performance liquid chromatography (HPLC). DBNs were stable in 10% FBS as well as 80% mouse serum for at least 8 h. To prepare Tc-99m-labeled DBNs, N-hydroxysuccinimidyl S-acetylmercaptoacetyl triglycinate (Sacetyl-MAG3-NHS ester) tagged single-stranded DNA (ssDNA) was first radiolabeled with Tc-99m, and DBNs with an overhang were assembled and then hybridized with Tc-99m-ssDNA to prepare Tc-99m-labeled DNA bipyramid nanostructures (Tc-99m-MAG3-DBNs). DBNs were radiolabeled, with the radiochemical purity being over 90%. The plasma half-life of Tc-99m-MAG3-DBNs in normal KM mice was about 6 min. The biodistribution and SPECT/CT imaging were conducted with Tc-99m-MAG3-DBNs in KM mice, and both showed that Tc-99m-MAG3-DBNs mainly concentrated in the intestine, liver, and kidneys, and there was also prominent uptake in the gallbladder and bladder. We successfully obtained a new class of SPECT molecular probes based on this DNA polyhedron structure.
收录类别	SCI
语种	英语
WOS记录号	WOS:000374812600008
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/25822]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
推荐引用方式 GB/T 7714	Li, JB,Jiang, DW,Bao, BL,et al. Radiolabeling of DNA Bipyramid and Preliminary Biological Evaluation in Mice[J]. BIOCONJUGATE CHEMISTRY,2016,27(4):905—910.
APA	Li, JB,Jiang, DW,Bao, BL,He, YL,Liu, L,&Wang, XM.(2016).Radiolabeling of DNA Bipyramid and Preliminary Biological Evaluation in Mice.BIOCONJUGATE CHEMISTRY,27(4),905—910.
MLA	Li, JB,et al."Radiolabeling of DNA Bipyramid and Preliminary Biological Evaluation in Mice".BIOCONJUGATE CHEMISTRY 27.4(2016):905—910.